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ATP1A1 Recombinant antibody, PBS Only

ATP1A1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240160E12

Cat no : 83191-6-PBS

Print datasheet

Synonyms

Na(+)/K(+) ATPase alpha-1 subunit, Na(+)/K(+) ATPase 1, EC:7.2.2.13, Atpa 1, ATP1A 1

验证数据展示

  • WB analysis using 83191-6-RR (same clone as 83191-6-PBS)

    WB analysis using 83191-6-RR (same clone as 83191-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83191-6-RR (ATP1A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83191-6-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 83191-6-RR (same clone as 83191-6-PBS)

    IF Staining of HEK-293 using 83191-6-RR (same clone as 83191-6-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ATP1A1 antibody (83191-6-RR, Clone: 240160E12 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83191-6-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 83191-6-RR (same clone as 83191-6-PBS)

    IF Staining of A431 using 83191-6-RR (same clone as 83191-6-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using ATP1A1 antibody (83191-6-RR, Clone: 240160E12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83191-6-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293 using 83191-6-RR (same clone as 83191-6-PBS)

    FC experiment of HEK-293 using 83191-6-RR (same clone as 83191-6-PBS)

    1x10^6 HEK-293 cells were intracellularly stained with 0.4 ug ATP1A1 Recombinant antibody (83191-6-RR, Clone:240160E12) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83191-6-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy ATP1A1 Ready-To-Use IHC Kit. ATP1A1 primary antibody included.

产品信息

83191-6-PBS targets ATP1A1 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen ATP1A1 fusion protein Ag5763 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ATPase, Na+/K+ transporting, alpha 1 polypeptide
Synonyms Na(+)/K(+) ATPase alpha-1 subunit, Na(+)/K(+) ATPase 1, EC:7.2.2.13, Atpa 1, ATP1A 1
Calculated Molecular Weight 113 kDa
Observed Molecular Weight 100-110 kDa
GenBank Accession NumberBC050359
Gene Symbol ATP1A1
Gene ID (NCBI) 476
ENSEMBL Gene IDENSG00000163399
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP05023
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ATP1A1 is the catalytic component of Na+/K+-ATPase which is a membrane bound enzyme primarily involved in generation of Na+ and K+ gradients across plasma membranes and in determination of cytoplasmic Na+ levels. ATP1A1 is a ubiquitously expressed membrane protein and often used as the marker or internal control for plasma membrane protein. For optimal WB detection of this membrane protein, we recommend to avoid boiling the sample after lysis.