CoraLite® Plus 647-conjugated ATP1A1 Recombinant antibody

ATP1A1 Recombinant Antibody for IF/ICC
Cat No. CL647-83191-6

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CloneNo. 240160E12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 647
Size: 

-/ -


经过测试的应用

Positive IF/ICC detected inA431 cells

For optimal WB detection with 83191-6-RR, we do not recommend boiling the sample after lysis.

Planning an IHC experiment? We recommend our IHCeasy ATP1A1 Ready-To-Use IHC Kit. ATP1A1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 83191-6-PBS

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-83191-6 targets ATP1A1 in IF/ICC applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse
Immunogen ATP1A1 fusion protein Ag5763 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ATPase, Na+/K+ transporting, alpha 1 polypeptide
Synonyms
Calculated Molecular Weight 113 kDa
Observed Molecular Weight 100-110 kDa
GenBank Accession NumberBC050359
Gene Symbol ATP1A1
Gene ID (NCBI) 476
ENSEMBL Gene IDENSG00000163399
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP05023
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATP1A1 is the catalytic component of Na+/K+-ATPase which is a membrane bound enzyme primarily involved in generation of Na+ and K+ gradients across plasma membranes and in determination of cytoplasmic Na+ levels. ATP1A1 is a ubiquitously expressed membrane protein and often used as the marker or internal control for plasma membrane protein. For optimal WB detection of this membrane protein, we recommend to avoid boiling the sample after lysis.

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 ATP1A1 antibody CL647-83191-6Download protocol
Standard Protocols
Click here to view our Standard Protocols
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