CoraLite® Plus 647-conjugated ATP1A1 Recombinant antibody

ATP1A1 Recombinant Antibody for IF/ICC
Cat No. CL647-83191-6

产品说明书

CloneNo. 240160E12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IF/ICC

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格: 

-/ -


经过测试的应用

Positive IF/ICC detected inA431 cells

For optimal WB detection with 83191-6-RR, we do not recommend boiling the sample after lysis.

Planning an IHC experiment? We recommend our IHCeasy ATP1A1 Ready-To-Use IHC Kit. ATP1A1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 83191-6-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-83191-6 targets ATP1A1 in IF/ICC applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse
免疫原 ATP1A1 fusion protein Ag5763 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ATPase, Na+/K+ transporting, alpha 1 polypeptide
别名
计算分子量 113 kDa
观测分子量 100-110 kDa
GenBank蛋白编号BC050359
基因名称 ATP1A1
Gene ID (NCBI) 476
ENSEMBL Gene IDENSG00000163399
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP05023
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATP1A1 is the catalytic component of Na+/K+-ATPase which is a membrane bound enzyme primarily involved in generation of Na+ and K+ gradients across plasma membranes and in determination of cytoplasmic Na+ levels. ATP1A1 is a ubiquitously expressed membrane protein and often used as the marker or internal control for plasma membrane protein. For optimal WB detection of this membrane protein, we recommend to avoid boiling the sample after lysis.

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 ATP1A1 antibody CL647-83191-6Download protocol
Standard Protocols
Click here to view our Standard Protocols
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