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COX4I2 Polyclonal antibody
COX4I2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HepG2 cells, human brain tissue, MCF-7 cells, human heart tissue, human lung tissue, A375 cells |
Positive IP detected in | HepG2 cells |
Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | Hela cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
11463-1-AP targets COX4I2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
Cited Applications | WB, IHC, IF |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Immunogen | COX4I2 fusion protein Ag1987 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | cytochrome c oxidase subunit IV isoform 2 (lung) |
Synonyms | COX4L2, COX4B, COX4 2, COX4, COX IV-2 |
Calculated Molecular Weight | 171 aa, 20 kDa |
Observed Molecular Weight | 17-18 kDa |
GenBank Accession Number | BC057779 |
Gene Symbol | COX4I2 |
Gene ID (NCBI) | 84701 |
RRID | AB_2085287 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q96KJ9 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for COX4I2 antibody 11463-1-AP | Download protocol |
IHC protocol for COX4I2 antibody 11463-1-AP | Download protocol |
IF protocol for COX4I2 antibody 11463-1-AP | Download protocol |
IP protocol for COX4I2 antibody 11463-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Rep Defective Mitochondrial Cardiolipin Remodeling Dampens HIF-1α Expression in Hypoxia. | ||
Oncotarget Nuclear-encoded cytochrome c oxidase subunit 4 regulates BMI1 expression and determines proliferative capacity of high-grade gliomas.
| ||
Sci Signal Acute O2 sensing through HIF2α-dependent expression of atypical cytochrome oxidase subunits in arterial chemoreceptors.
| ||
Free Radic Biol Med Cytochrome c oxidase mediates labile iron level and radioresistance in glioblastoma. | ||
Bioconjug Chem GnRH-Gemcitabine Conjugates for the Treatment of Androgen-Independent Prostate Cancer: Pharmacokinetic Enhancements Combined with Targeted Drug Delivery. | ||
Free Radic Biol Med Differentiation of SH-SY5Y cells to a neuronal phenotype changes cellular bioenergetics and the response to oxidative stress. |