- Featured Product
- KD/KO Validated
COX4I2 Polyclonal antibody
COX4I2 Polyclonal Antibody for IF, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF, IHC, IP, WB,ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, human brain tissue, MCF-7 cells, human heart tissue, human lung tissue, A375 cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | Hela cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| Sample-dependent, check data in validation data gallery | |
产品信息
11463-1-AP targets COX4I2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF, IHC, IP, WB,ELISA |
| Cited Applications | IF, IHC, WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | COX4I2 fusion protein Ag1987 |
| Full Name | cytochrome c oxidase subunit IV isoform 2 (lung) |
| Calculated molecular weight | 171 aa, 20 kDa |
| Observed molecular weight | 17-18 kDa |
| GenBank accession number | BC057779 |
| Gene symbol | COX4I2 |
| Uniprot | Q96KJ9 | Gene ID (NCBI) | 84701 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
COX4I2, also named as COX4L2 and COXIV-2, belongs to the cytochrome c oxidase IV family. It is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. COXIV(cytochrome c oxidase IV) has two isoforms (isoform 1 and 2). Isoform 1(COX4I1) is ubiquitously expressed and isoform 2 is highly expressed in lung tissues. This antibody was generated against full length COX4I2 protein.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for COX4I2 antibody 11463-1-AP | Download protocol |
| IHC protocol for COX4I2 antibody 11463-1-AP | Download protocol |
| IF protocol for COX4I2 antibody 11463-1-AP | Download protocol |
| IP protocol for COX4I2 antibody 11463-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Bioconjug Chem GnRH-Gemcitabine Conjugates for the Treatment of Androgen-Independent Prostate Cancer: Pharmacokinetic Enhancements Combined with Targeted Drug Delivery. | ||
J Mol Endocrinol Decreased mixed lineage leukemia 1 is involved in endometriosis-related infertility. | ||
Am J Physiol Heart Circ Physiol Type II diabetes increases mitochondrial DNA mutations in the left ventricle of the Goto-Kakizaki diabetic rat. | ||
Biochim Biophys Acta Abl2 kinase phosphorylates Bi-organellar regulator MNRR1 in mitochondria, stimulating respiration. | ||
J Physiol Gene expression analyses reveal metabolic specifications in acute O2 -sensing chemoreceptor cells. |
