|Positive WB detected in||HepG2 cells, human brain tissue, MCF-7 cells, human heart tissue, human lung tissue, A375 cells|
|Positive IP detected in||HepG2 cells|
|Positive IHC detected in||human ovary tumor tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||Hela cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
11463-1-AP targets COX4I2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Applications||IF, IHC, IP, WB,ELISA|
|Cited Applications||IF, IHC, WB|
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||COX4I2 fusion protein Ag1987|
|Full Name||cytochrome c oxidase subunit IV isoform 2 (lung)|
|Calculated molecular weight||171 aa, 20 kDa|
|Observed molecular weight||17-18 kDa|
|GenBank accession number||BC057779|
|Uniprot||Q96KJ9||Gene ID (NCBI)||84701|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
COX4I2, also named as COX4L2 and COXIV-2, belongs to the cytochrome c oxidase IV family. It is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. COXIV(cytochrome c oxidase IV) has two isoforms (isoform 1 and 2). Isoform 1(COX4I1) is ubiquitously expressed and isoform 2 is highly expressed in lung tissues. This antibody was generated against full length COX4I2 protein.
|Product Specific Protocols|
|WB protocol for COX4I2 antibody 11463-1-AP||Download protocol|
|IHC protocol for COX4I2 antibody 11463-1-AP||Download protocol|
|IF protocol for COX4I2 antibody 11463-1-AP||Download protocol|
|IP protocol for COX4I2 antibody 11463-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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