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COX4I2 Polyclonal antibody

COX4I2 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, IHC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 11463-1-AP

Print datasheet

Synonyms

COX IV 2, COX4, COX4 2, COX4B, COX4I2, COX4L2, COXIV 2



经过测试的应用

Positive WB detected inHepG2 cells, human brain tissue, MCF-7 cells, human heart tissue, human lung tissue, A375 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

产品信息

11463-1-AP targets COX4I2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF, IHC, IP, WB,ELISA
Cited Applications IF, IHC, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen COX4I2 fusion protein Ag1987
Full Name cytochrome c oxidase subunit IV isoform 2 (lung)
Calculated molecular weight 171 aa, 20 kDa
Observed molecular weight 17-18 kDa
GenBank accession numberBC057779
Gene symbol COX4I2
UniprotQ96KJ9
Gene ID (NCBI) 84701
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

COX4I2, also named as COX4L2 and COXIV-2, belongs to the cytochrome c oxidase IV family. It is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. COXIV(cytochrome c oxidase IV) has two isoforms (isoform 1 and 2). Isoform 1(COX4I1) is ubiquitously expressed and isoform 2 is highly expressed in lung tissues. This antibody was generated against full length COX4I2 protein.

实验方案

Product Specific Protocols
WB protocol for COX4I2 antibody 11463-1-APDownload protocol
IHC protocol for COX4I2 antibody 11463-1-APDownload protocol
IF protocol for COX4I2 antibody 11463-1-APDownload protocol
IP protocol for COX4I2 antibody 11463-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Bioconjug Chem

GnRH-Gemcitabine Conjugates for the Treatment of Androgen-Independent Prostate Cancer: Pharmacokinetic Enhancements Combined with Targeted Drug Delivery.

Authors - Theodoros Karampelas
humanIHC, WB

J Mol Endocrinol

Decreased mixed lineage leukemia 1 is involved in endometriosis-related infertility.

Authors - Xue Wen
ratWB

Am J Physiol Heart Circ Physiol

Type II diabetes increases mitochondrial DNA mutations in the left ventricle of the Goto-Kakizaki diabetic rat.

Authors - Hicks S S
humanWB

Biochim Biophys Acta

Abl2 kinase phosphorylates Bi-organellar regulator MNRR1 in mitochondria, stimulating respiration.

Authors - Siddhesh Aras
humanWB

J Biol Chem

PMID: 20870728

Authors - Oliva Claudia R CR
mouseIF

J Physiol

Gene expression analyses reveal metabolic specifications in acute O2 -sensing chemoreceptor cells.

Authors - Lin Gao