Beta Tubulin Polyclonal antibody

Beta Tubulin Polyclonal Antibody for FC, IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (6)





Cat No : 10068-1-AP

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beta 4, beta tubulin, B-tubulin, MC1R, TUBB, tubulin


Positive WB detected inmouse cerebellum tissue, HeLa cells, mouse brain tissue, rat brain tissue, HEK-293 cells, Jurkat cells, HepG2 cells, A431 cells, NIH/3T3 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inmouse brain tissue, human brain tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inC2C12 cells, HepG2 cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy TUBB3 Ready-To-Use IHC Kit. TUBB3 primary antibody included.
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.


Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.


10068-1-AP targets Beta Tubulin in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IF/ICC, IHC, IP, WB, ELISA
Cited ApplicationsWB, IP, IF, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, zebrafish, pig, Caenorhabditis elegans, canine, silkworm, bovine
Immunogen Beta Tubulin fusion protein Ag0117 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tubulin, beta 3
Synonyms beta 4, beta tubulin, B-tubulin, MC1R, TUBB, tubulin
Calculated Molecular Weight 450 aa, 50 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC000748
Gene Symbol TUBB3
Gene ID (NCBI) 10381
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.


There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart, once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha, beta, and gamma tubulins have all been used as loading controls. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs.


Product Specific Protocols
WB protocol for Beta Tubulin antibody 10068-1-APDownload protocol
IHC protocol for Beta Tubulin antibody 10068-1-APDownload protocol
IF protocol for Beta Tubulin antibody 10068-1-APDownload protocol
IP protocol for Beta Tubulin antibody 10068-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




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