Beta Tubulin Polyclonal antibody
Beta Tubulin Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (7)
Applications
FC, IF, IHC, IP, WB, ELISA
Conjugate
Unconjugated
252
验证数据展示
经过测试的应用
| Positive WB detected in | mouse cerebellum tissue, HeLa cells, mouse brain tissue, rat brain tissue, HEK-293 cells, Jurkat cells, HepG2 cells, A431 cells, NIH/3T3 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | mouse brain tissue, human brain tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | C2C12 cells, HepG2 cells |
| Positive FC detected in | HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:2000-1:10000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| Sample-dependent, check data in validation data gallery | |
产品信息
10068-1-AP targets Beta Tubulin in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | FC, IF, IHC, IP, WB, ELISA |
| Cited Applications | IF, IHC, IP, WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, bovine, Caenorhabditis elegans, canine, mosue, pig, silkworm, zebrafish |
| Immunogen | Beta Tubulin fusion protein Ag0117 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | tubulin, beta 3 |
| Synonyms | beta 4, beta tubulin, B-tubulin, MC1R, TUBB, tubulin |
| Calculated molecular weight | 450 aa, 50 kDa |
| Observed molecular weight | 50-55 kDa |
| GenBank accession number | BC000748 |
| Gene symbol | TUBB3 |
| Uniprot | Q13509 |
| Gene ID (NCBI) | 10381 |
| RRID | AB_2303998 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart, once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha, beta, and gamma tubulins have all been used as loading controls. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Beta Tubulin antibody 10068-1-AP | Download protocol |
| IHC protocol for Beta Tubulin antibody 10068-1-AP | Download protocol |
| IF protocol for Beta Tubulin antibody 10068-1-AP | Download protocol |
| IP protocol for Beta Tubulin antibody 10068-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Complement Signals Determine Opposite Effects of B Cells in Chemotherapy-Induced Immunity. | ||
Sci Adv Loss of schizophrenia-related miR-501-3p in mice impairs sociability and memory by enhancing mGluR5-mediated glutamatergic transmission | ||
Sci Adv PKM2 promotes pulmonary fibrosis by stabilizing TGF-β1 receptor I and enhancing TGF-β1 signaling | ||
Nat Commun Identification of predictors of drug sensitivity using patient-derived models of esophageal squamous cell carcinoma. | ||
Nat Commun Second messenger Ap4A polymerizes target protein HINT1 to transduce signals in FcεRI-activated mast cells. | ||
Nucleic Acids Res Activation of bivalent factor DLX5 cooperates with master regulator TP63 to promote squamous cell carcinoma. |
