Beta Tubulin Polyclonal antibody

Beta Tubulin Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 10068-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (6)

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (1)

Tubulin beta III, Tubulin beta 3 chain, Tubulin beta-III, Tubulin beta-4 chain, Tubulin beta-3 chain

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite®594
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse cerebellum tissue, HeLa cells, mouse brain tissue, rat brain tissue, HEK-293 cells, Jurkat cells, HepG2 cells, A431 cells, NIH/3T3 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inmouse brain tissue, human brain tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inC2C12 cells, HepG2 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy TUBB3 Ready-To-Use IHC Kit. TUBB3 primary antibody included.
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10068-1-AP targets Beta Tubulin in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, pig, canine, zebrafish, bovine, caenorhabditis elegans, silkworm
免疫原 Beta Tubulin fusion protein Ag0117 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tubulin, beta 3
别名 Tubulin beta III, Tubulin beta 3 chain, Tubulin beta-III, Tubulin beta-4 chain, Tubulin beta-3 chain
计算分子量 450 aa, 50 kDa
观测分子量 50-55 kDa
GenBank蛋白编号BC000748
基因名称 TUBB3
Gene ID (NCBI) 10381
RRIDAB_2303998
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13509
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart, once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha, beta, and gamma tubulins have all been used as loading controls. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs.

实验方案

Product Specific Protocols
WB protocol for Beta Tubulin antibody 10068-1-APDownload protocol
IHC protocol for Beta Tubulin antibody 10068-1-APDownload protocol
IF protocol for Beta Tubulin antibody 10068-1-APDownload protocol
IP protocol for Beta Tubulin antibody 10068-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell

Complement Signals Determine Opposite Effects of B Cells in Chemotherapy-Induced Immunity.

Authors - Yiwen Lu
humanWB

Nat Struct Mol Biol

CRISPR-Cas9-based functional interrogation of unconventional translatome reveals human cancer dependency on cryptic non-canonical open reading frames

Authors - Caishang Zheng
WB

Nat Commun

Gonococcal OMV-delivered PorB induces epithelial cell mitophagy

Authors - Shuai Gao
humanWB

Nat Commun

MYG1 drives glycolysis and colorectal cancer development through nuclear-mitochondrial collaboration

Authors - Jianxiong Chen
WB

Adv Sci (Weinh)

EGFR-Induced and c-Src-Mediated CD47 Phosphorylation Inhibits TRIM21-Dependent Polyubiquitylation and Degradation of CD47 to Promote Tumor Immune Evasion

Authors - Linyong Du
mouseWB

Acta Pharm Sin B

Nuciferine protects against high-fat diet-induced hepatic steatosis and insulin resistance via activating TFEB-mediated autophagy-lysosomal pathway.

Authors - Xiliang Du
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