CoraLite®594-conjugated Beta Tubulin Polyclonal antibody

Beta Tubulin Polyclonal Antibody for FC, IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC, IF

Conjugate

CoraLite®594 Fluorescent Dye

Cat No : CL594-10068

Print datasheet

Synonyms

beta 4, beta tubulin, MC1R, TUBB, TUBB3, TUBB4, Tubulin beta 3 chain, Tubulin beta 4 chain, Tubulin beta III, tubulin, beta 3



经过测试的应用

Positive IF detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy TUBB3 Ready-To-Use IHC Kit. TUBB3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10068-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-10068 targets Beta Tubulin in IF, FC applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IF
Tested Reactivity human, mouse, rat
Immunogen Beta Tubulin fusion protein Ag0117 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tubulin, beta 3
Synonyms beta 4, beta tubulin, MC1R, TUBB, TUBB3, TUBB4, Tubulin beta 3 chain, Tubulin beta 4 chain, Tubulin beta III, tubulin, beta 3
Calculated Molecular Weight 450 aa, 50 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC000748
Gene Symbol TUBB3
Gene ID (NCBI) 10381
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13509
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart, once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha, beta, and gamma tubulins have all been used as loading controls. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs.

实验方案

Product Specific Protocols
IF protocol for CL594 Beta Tubulin antibody CL594-10068Download protocol
FC protocol for CL594 Beta Tubulin antibody CL594-10068Download protocol
Standard Protocols
Click here to view our Standard Protocols