DYKDDDDK tag Polyclonal antibody (Binds to FLAG® tag epitope)

DYKDDDDK tag Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG


recombinant protein and More (7)


IF, WB,ELISA and More (6)



Cat No : 20543-1-AP

Print datasheet




Positive WB detected inrecombinant protein
Positive IF detected inTransfected HEK-293 cells


Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery


The immunogen of 20543-1-AP is DYKDDDDK tag Fusion Protein expressed in E. coli.

Tested Applications IF, WB,ELISA
Cited Applications ChIP, CoIP, FC, IF, IHC, IP, RIP, WB
Tested Reactivity recombinant protein
Cited Reactivityhuman, mouse, arabidopsis, chicken, D. punctatus, pig, Plant
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DYKDDDDK tag fusion protein Ag2329
Full Name DYKDDDDK tag
Gene symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.


Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal DDDDK vectors provide an Ek cleavage site for removal of the fusion tag. The DDDDK peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the DDDDK peptide is more likely to be accessible to antibodies. A DDDDK-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on. This antibody is a rabbit polyclonal antibody raised against 3xFlag (3xDYKDDDDKT) sequence and recognizes the (1x) and (3x)DYKDDDDK peptide and detects DDDDK-tagged proteins. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.


Product Specific Protocols
WB protocol for DYKDDDDK tag antibody 20543-1-APDownload protocol
IF protocol for DYKDDDDK tag antibody 20543-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



J Biol Chem

Retractile lysyl-tRNA synthetase-AIMP2 assembly in the human multi-aminoacyl-tRNA synthetase complex.

Authors - Zhoufei Hei


A gene-based recessive diplotype exome scan discovers FGF6, a novel hepcidin-regulating iron metabolism gene.

Authors - Shicheng Guo

J Virol

The extended C-terminal region of influenza C nucleoprotein is important for nuclear import and RNP activity.

Authors - Yun-Sang Tang

Nat Commun

Tyrosine phosphorylation activates 6-phosphogluconate dehydrogenase and promotes tumor growth and radiation resistance.

Authors - Ruilong Liu

Dev Cell

FGF15 Activates Hippo Signaling to Suppress Bile Acid Metabolism and Liver Tumorigenesis.

Authors - Suyuan Ji

Sci Adv

The endogenous retrovirus-derived long noncoding RNA TROJAN promotes triple-negative breast cancer progression via ZMYND8 degradation.

Authors - Xi Jin