DYKDDDDK tag Polyclonal antibody (Binds to FLAG® tag epitope)

DYKDDDDK tag Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

recombinant protein and More (7)

Applications

IF, WB,ELISA and More (6)

Conjugate

Unconjugated

Cat No : 20543-1-AP

Print datasheet

Synonyms

DDDDK tag, DYKDDDDK, Flag



经过测试的应用

Positive WB detected inrecombinant protein
Positive IF detected inTransfected HEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

产品信息

The immunogen of 20543-1-AP is DYKDDDDK tag Fusion Protein expressed in E. coli.

Tested Applications IF, WB,ELISA
Cited Applications ChIP, CoIP, FC, IF, IHC, IP, RIP, WB
Tested Reactivity recombinant protein
Cited Reactivityhuman, mouse, arabidopsis, chicken, D. punctatus, pig, Plant
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DYKDDDDK tag fusion protein Ag2329
Full Name DYKDDDDK tag
Gene symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal DDDDK vectors provide an Ek cleavage site for removal of the fusion tag. The DDDDK peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the DDDDK peptide is more likely to be accessible to antibodies. A DDDDK-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on. This antibody is a rabbit polyclonal antibody raised against 3xFlag (3xDYKDDDDKT) sequence and recognizes the (1x) and (3x)DYKDDDDK peptide and detects DDDDK-tagged proteins. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.

实验方案

Product Specific Protocols
WB protocol for DYKDDDDK tag antibody 20543-1-APDownload protocol
IF protocol for DYKDDDDK tag antibody 20543-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

J Biol Chem

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J Virol

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Nat Commun

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Sci Adv

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