CoraLite® Plus 750-conjugated DYKDDDDK tag Polyclonal antibody (Binds to FLAG® tag epitope)

DYKDDDDK tag Polyclonal Antibody for WB

Host / Isotype

Rabbit / IgG

Reactivity

recombinant protein

Applications

WB

Conjugate

CoraLite® Plus 750 Fluorescent Dye

Cat no : CL750-20543

Print datasheet

Synonyms

DDDDK, DDDDK tag, DYKDDDDK, DYKDDDDK tag, Flag



经过测试的应用

Positive WB detected inTransfected HEK-293 cells
For other applications, we recommend the unconjugated version of this antibody, 20543-1-AP

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL750-20543 targets DYKDDDDK tag in WB applications and shows reactivity with recombinant protein samples.

Tested Applications WB Application Description
Tested Reactivity recombinant protein
Immunogen DYKDDDDK tag fusion protein Ag2329 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name DYKDDDDK tag
Synonyms DDDDK, DDDDK tag, DYKDDDDK, DYKDDDDK tag, Flag
Gene Symbol
Gene ID (NCBI)
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission Maxima Wavelengths755 nm / 780 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal DDDDK vectors provide an Ek cleavage site for removal of the fusion tag. The DDDDK peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the DDDDK peptide is more likely to be accessible to antibodies. A DDDDK-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on. This antibody is a rabbit polyclonal antibody raised against 3xFlag (3xDYKDDDDKT) sequence and recognizes the (1x) and (3x)DYKDDDDK peptide and detects DDDDK-tagged proteins. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.

实验方案

Product Specific Protocols
WB protocol for CL Plus 750 DYKDDDDK tag antibody CL750-20543Download protocol
Standard Protocols
Click here to view our Standard Protocols