CoraLite® Plus 750-conjugated DYKDDDDK tag Polyclonal antibody (Binds to FLAG® tag epitope)

DYKDDDDK tag Polyclonal Antibody for WB, FC (Intra)
Cat No. CL750-20543

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

recombinant protein

应用

WB, FC (Intra)

Flag, DYKDDDDK, DDDDK tag, DDDDK

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 750
规格: 

-/ -


经过测试的应用

Positive WB detected inTransfected HEK-293 cells
Positive FC (Intra) detected inTransfected HEK-293 cells
For other applications, we recommend the unconjugated version of this antibody, 20543-1-AP

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL750-20543 targets DYKDDDDK tag in WB, FC (Intra) applications and shows reactivity with recombinant protein samples.

经测试应用 WB, FC (Intra) Application Description
经测试反应性 recombinant protein
免疫原 DYKDDDDK tag fusion protein Ag2329 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Flag Tag
别名 Flag, DYKDDDDK, DDDDK tag, DDDDK
基因名称 Flag Tag
Gene ID (NCBI) 8
RRIDAB_3673693
偶联类型 CoraLite® Plus 750 Fluorescent Dye
最大激发/发射波长755 nm / 780 nm
形式 Liquid
纯化方式Antigen affinity purification
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal DDDDK vectors provide an Ek cleavage site for removal of the fusion tag. The DDDDK peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the DDDDK peptide is more likely to be accessible to antibodies. A DDDDK-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on. This antibody is a rabbit polyclonal antibody raised against 3xFlag (3xDYKDDDDKT) sequence and recognizes the (1x) and (3x)DYKDDDDK peptide and detects DDDDK-tagged proteins. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.

实验方案

Product Specific Protocols
WB protocol for CL Plus 750 DYKDDDDK tag antibody CL750-20543Download protocol
FC protocol for CL Plus 750 DYKDDDDK tag antibody CL750-20543Download protocol
Standard Protocols
Click here to view our Standard Protocols
Loading...