CoraLite® Plus 750-conjugated DYKDDDDK tag Polyclonal antibody (Binds to FLAG® tag epitope)
DYKDDDDK tag Polyclonal Antibody for WB
Host / Isotype
Rabbit / IgG
Reactivity
recombinant protein
Applications
WB
Conjugate
CoraLite® Plus 750 Fluorescent Dye
验证数据展示
经过测试的应用
Positive WB detected in | Transfected HEK-293 cells |
For other applications, we recommend the unconjugated version of this antibody, 20543-1-AP
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:2000-1:16000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL750-20543 targets DYKDDDDK tag in WB applications and shows reactivity with recombinant protein samples.
Tested Applications | WB Application Description |
Tested Reactivity | recombinant protein |
Immunogen | DYKDDDDK tag fusion protein Ag2329 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | DYKDDDDK tag |
Synonyms | DDDDK, DDDDK tag, DYKDDDDK, DYKDDDDK tag, Flag |
Gene Symbol | |
Gene ID (NCBI) | |
Conjugate | CoraLite® Plus 750 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 755 nm / 780 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal DDDDK vectors provide an Ek cleavage site for removal of the fusion tag. The DDDDK peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the DDDDK peptide is more likely to be accessible to antibodies. A DDDDK-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on. This antibody is a rabbit polyclonal antibody raised against 3xFlag (3xDYKDDDDKT) sequence and recognizes the (1x) and (3x)DYKDDDDK peptide and detects DDDDK-tagged proteins. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.
实验方案
Product Specific Protocols | |
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WB protocol for CL Plus 750 DYKDDDDK tag antibody CL750-20543 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |