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AbFlex HA-Tag antibody (rAb)

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Not Species Specific

Applications

IP

Cat No : 91271,91272 91271

Synonyms

验证数据展示

  • AbFlex HA-Tag antibody (rAb) tested by Immunoprecipitation. Whole-cell lysate (400 ug) from U2OS cells expressing an HA-Tagged protein, was immunoprecipitated with 2 ug of AbFlex HA-Tag rAb (lane 1) or control IgG2a (lane 2). Immunoprecipitates were run on SDS-PAGE and analyzed by Western blot using HA-Tag mAb Cat. No. 39627 at 4 ug/ml.

    AbFlex HA-Tag antibody (rAb) tested by Immunoprecipitation. Whole-cell lysate (400 ug) from U2OS cells expressing an HA-Tagged protein, was immunoprecipitated with 2 ug of AbFlex HA-Tag rAb (lane 1) or control IgG2a (lane 2). Immunoprecipitates were run on SDS-PAGE and analyzed by Western blot using HA-Tag mAb Cat. No. 39627 at 4 ug/ml.

产品信息

Tested Applications IP

Applications Validated by Active Motif: IP: 2 - 5 ug per IP AbFlex recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone, see Catalog No. 39627.
Tested Reactivity Human, Not Species Specific
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
ModificationHA-Tag
Immunogen This antibody was raised against a peptide containing multiple repeats of the HA tag epitope YPYDVPDYA.
Full Name AbFlex HA-Tag antibody (rAb)
Synonyms
Molecular weight
GenBank accession numberN/A
RRIDAB_2793828
Purification Method Protein A Chromatography
Buffer Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035% sodium azide, 30% glycerol. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

背景介绍

AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex HA-Tag antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. HA-Tag has been derived from the hemagglutinin protein of the human influenza virus for use as an epitope for the detection and purification of tagged recombinant proteins expressed in vivo. The HA-Tag is a small epitope and generally does not interfere with the normal function of recombinant proteins.