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AbFlex HDAC2 antibody (rAb)

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

ChIP-Seq, WB

Cat No : 91197,91198 91197

Synonyms

验证数据展示

  • AbFlex HDAC2 recombinant antibody (rAb) tested by ChIP-Seq Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with 30 ug of chromatin from NCI-H1437 (human lung carcinoma cell line) chromatin and 4 ug HDAC2 antibody. ChIP DNA was sequenced on the Illumina HiSeq and 15.6 million sequence tags were mapped to identify HDAC2 binding sites on chromosome 1.

    AbFlex HDAC2 recombinant antibody (rAb) tested by ChIP-Seq Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with 30 ug of chromatin from NCI-H1437 (human lung carcinoma cell line) chromatin and 4 ug HDAC2 antibody. ChIP DNA was sequenced on the Illumina HiSeq and 15.6 million sequence tags were mapped to identify HDAC2 binding sites on chromosome 1.

  • Western blot of AbFlex HDAC2 antibody (rAb). 20 ug HeLa cell nuclear extract was run on SDS-PAGE and probed with 0.5 ug/ml AbFlex HDAC2 antibody (rAb). MW: 55 kDa

    Western blot of AbFlex HDAC2 antibody (rAb). 20 ug HeLa cell nuclear extract was run on SDS-PAGE and probed with 0.5 ug/ml AbFlex HDAC2 antibody (rAb). MW: 55 kDa

产品信息

Tested Applications ChIP-Seq, WB

Applications Validated by Active Motif: ChIP-Seq: 4 - 10 ug per ChIP WB*: 0.2-2 ug/ml For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background. Individual optimization may be required. *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.
Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Immunogen This antibody was raised against peptide corresponding to amino acids 473-488 of human HDAC2.
Full Name AbFlex HDAC2 antibody (rAb)
Synonyms
Molecular weight 55 kDa
GenBank accession numberNP_001518
RRIDAB_2793799
Purification Method Protein A Chromatography
Buffer Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 35 mM NaOAc, 0.07% sodium azide, 30% glycerol. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

背景介绍

AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. HDAC2 (Histone Deacetylase 2, also designated mammalian RPD3) is a member of the class I mammalian histone deacetylases (HDACs) involved in regulating chromatin structure during transcription. These enzymes catalyze the removal of acetyl groups from lysine residues of histones and other cellular proteins. Lysine N-e-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in regulation of gene expression in various cellular functions. It consists of the transfer of an acetyl moiety from an acetyl coenzyme A to the e-amino group of a lysine residue. In vivo, acetylation is controlled by the antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs). The HDACs are grouped into four classes, on the basis of similarity to yeast counterparts: HDAC class I (HDAC1, HDAC2, HDAC3 and HDAC8), class II (HDAC4, HDAC5, HDAC6, HDAC7, HDAC9 and HDAC10), class III (SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6 and SIRT-7) and class IV (HDAC11). HDAC2 is associated with many different proteins as YY1 (a mammalian zinc-finger transcription factor). HDAC2 also forms transcriptional repressor complexes containing, among others, HDAC1 or RBBP4. HDAC1, HDAC2 and HDAC3 are also ubiquitously expressed and can deacetylate both Histone H3 and Histone H4 in free histones or nucleosome substrate.