AbFlex Histone H3K79me2 antibody   (rAb)

Host / Isotype

Rabbit / IgG2a

Reactivity

Human, Wide Range Predicted

Applications

ChIP, DB, WB

Cat No : 91399,91400 91399

Synonyms

H3 trimethyl-lys79, Histone H3 dimethyl-lys79, dimethylated lysine 79, trimethylated lysine 79 of histone h3, K79, dmk79, tmk79, histone, histone h3, chromatin, histone methylation, nucleosome, western blotting, wb, polyclonal, pab, antibody, sample



产品信息

Tested Applications ChIP, DB, WB

Applications Validated by Active Motif: ChIP-Seq: 4 ug per ChIP WB: 0.5 - 2 ug/ml dilution DB: 1 - 2 ug/ml dilution

Tested Reactivity Human, Wide Range Predicted
Host / Isotype Rabbit / IgG2a
Class Recombinant
Type Antibody
ModificationMethylated
Immunogen This Histone H3 dimethyl Lys79 antibody was raised against a peptide including dimethyl-lysine 79 of histone H3.
Full Name AbFlex Histone H3K79me2 antibody   (rAb)
Synonyms H3 trimethyl-lys79, Histone H3 dimethyl-lys79, dimethylated lysine 79, trimethylated lysine 79 of histone h3, K79, dmk79, tmk79, histone, histone h3, chromatin, histone methylation, nucleosome, western blotting, wb, polyclonal, pab, antibody, sample
Molecular weight 17 kDa
GenBank accession numberNP_003520
Purification Method Protein A Chromatography
Buffer 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035% sodium azide, and 30% glycerol.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

背景介绍

AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex Histone H3K79me2 antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. Lysine 79 of histone H3 can be mono-, di- or trimethylated by Dot1 methylase; methylation at this residue acts as a marker of inactive chromatin regions that is critical for transcriptional silencing, and it is thought that silencing proteins such as Sir3 function by blocking Dot1 methylation.