• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

AbFlex KDM1B / LSD2 antibody (rAb)

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB

Cat No : 91327,91328 91327

Synonyms

KDM1B / LSD2, Lysine (K)-Specific Demethylase 1B, Flavin-Containing Amine Oxidase Domain-Containing Protein 1, AOF1, Lysine-Specific Histone Demethylase 2, LSD2, LSD, abflex, recombinant antibody, KDM,

验证数据展示

  • AbFlex KDM1B / LSD2 antibody tested by Western blot. 20 ug of K562 nuclear extract* was run on SDS-PAGE and probed with AbFlex KDM1B / LSD2 antibody at 0.5 ug/ml.

    AbFlex KDM1B / LSD2 antibody tested by Western blot. 20 ug of K562 nuclear extract* was run on SDS-PAGE and probed with AbFlex KDM1B / LSD2 antibody at 0.5 ug/ml.

产品信息

Tested Applications WB

Applications Validated by Active Motif: WB*: 0.5 - 2 ug/ml *Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot. For optimal results, we also recommend the addition of 0.05% Tween-20 to all blocking solutions to reduce background. Individual optimization may be required.
Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Immunogen This antibody was raised against full length human LKDM1B / LSD2 recombinant protein.
Full Name AbFlex KDM1B / LSD2 antibody (rAb)
Synonyms KDM1B / LSD2, Lysine (K)-Specific Demethylase 1B, Flavin-Containing Amine Oxidase Domain-Containing Protein 1, AOF1, Lysine-Specific Histone Demethylase 2, LSD2, LSD, abflex, recombinant antibody, KDM,
Molecular weight Predicted: 93kDa / Observed: 105 kDa
GenBank accession numberNP_694587
Purification Method Protein A Chromatography
Buffer 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035% sodium azide, and 30% glycerol. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

背景介绍

AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex KDM1B / LSD2 was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in CHO cells. KDM1B / LSD2 (Lysine (K)-Specific Demethylase 1B / Lysine-Specific Histone Demethylase 2) is a histone demethylase that demethylates Lys4 of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Required for de novo DNA methylation of a subset of imprinted genes during oogenesis. Demethylates both mono- and di-methylated Lys4 of histone H3. Has no effect on tri-methylated Lys4, mono-, di- or tri-methylated Lys9, mono-, di- or tri-methylated Lys27, mono-, di- or tri-methylated Lys36 of histone H3, or on mono-, di- or tri-methylated Lys20 of histone H4 (By similarity).