AbFlex Mili / PiwiL2 antibody (rAb)
Host / Isotype
Mouse / IgG2a
Reactivity
Mouse
Applications
WB
Cat No : 91253,91254 91253
Synonyms
验证数据展示
产品信息
| Tested Applications |
WB
Applications Validated by Active Motif: WB: 0.2 - 2 ug/ml AbFlex recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone, see Catalog No. 61143. |
| Tested Reactivity | Mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | This antibody was raised against full length mouse MILI / PIWIL2 (Miwi like / piwi-like 2) recombinant protein. |
| Full Name | AbFlex Mili / PiwiL2 antibody (rAb) |
| Synonyms | Mili, PiwiL2, PIWI, AGO, piwi-interacting RNA, piRNA, transposons, methylation, retrotranposon, spermatogenesis, germ line stem cell division, testis, differentiation, recombinant antibodies |
| Molecular weight | 110 kDa |
| GenBank accession number | NP_001129193 | RRID | AB_2793820 | Purification Method | Protein A Chromatography |
| Buffer | Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035% sodium azide, 30% glycerol. Sodium azide is highly toxic. |
| Storage | Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage. |
背景介绍
AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex Mili / PiwiL2 antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. Mili / PiwiL2 is a member of the PIWI/AGO subfamily of RNA-binding proteins. Mili / PiwiL2 contains a Piwi and an AGO domain and binds a class of RNA called piwi-interacting RNA (piRNA), which are 24 to 30 nucleotides in length and are derived primarily derived from transposons and other repeated sequence elements. Mili / PiwiL2 is required for methylation and inactivation of retrotransposons. Mice deficient in Mili / PiwiL2 are sterile, as it is also required for spermatogenesis. It also positively regulates translation, and is required for germ line stem cell division and differentiation.