AbFlex N6-Methyladenosine (m6A) antibody (rAb)
Host / Isotype
Mouse / IgG2a
Reactivity
Human, Mouse, Not Species Specific
Applications
DB, IP
Cat No : 91261,91262 91261
Synonyms
验证数据展示
产品信息
Tested Applications |
DB, IP
Applications Validated by Active Motif: DB IP: 5 - 10 ug per IP |
Tested Reactivity | Human, Mouse, Not Species Specific |
Host / Isotype | Mouse / IgG2a |
Class | Recombinant |
Type | Antibody |
Modification | Methylated |
Immunogen | This antibody was raised against N6-methyladenosine conjugated to BSA. |
Full Name | AbFlex N6-Methyladenosine (m6A) antibody (rAb) |
Synonyms | N6-Metheyladenosine, m6A, methyladenosine, AbFlex, recombinant antibody |
Molecular weight | |
GenBank accession number | N/A | Purification Method | Protein A Chromatography |
Buffer | Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035% sodium azide, 30% glycerol. Sodium azide is highly toxic. |
Storage | Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage. |
背景介绍
AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex N6-Methyladenosine (m6A) antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. N6-Methyladenosine (m6A) is an RNA modification on the N-6 position of adenosine. This modification has been found to be abundant in the 3’ UTR and stop codons of mammalian mRNA. m6A is associated with miRNA binding sites suggesting a potential role in epigenetic gene regulation. FTO and ALKBH are demethylases for 6-methyladenosine while a multiprotein complex that includes METTL3 functions as the methyltransferase. Recent findings revealed that m6A is also present on metazoan DNA, suggesting a genuine epigenetic role for this modification in the context of DNA as well.