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Caspase-8 antibody (mAb) (Clone 90A992)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB

CloneNo.

90A992

Cat No : 40930 40930

Synonyms

Caspase-8, Caspase8, Caspase 8, mAb, monoclonal, CASP8, apoptosis-related cysteine peptidase 8, antibody, antibodies

验证数据展示

  • Caspase-8 mAb tested by Western blot. Caspase-8 detection by Western blot. The analysis was performed using Jurkat extracts with Caspase-8 mAb at 2 ug/ml (lane 1) and 0.5 ug/ml (lane 2) dilutions. The antibody only detects 55 kDa caspase-8 in staurosporine-treated (2 hours) Jurkat cells.

    Caspase-8 mAb tested by Western blot. Caspase-8 detection by Western blot. The analysis was performed using Jurkat extracts with Caspase-8 mAb at 2 ug/ml (lane 1) and 0.5 ug/ml (lane 2) dilutions. The antibody only detects 55 kDa caspase-8 in staurosporine-treated (2 hours) Jurkat cells.

产品信息

Tested Applications WB

Applications Validated by Active Motif: WB: 1 - 2 ug/ml dilution For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background. Individual optimization may be required.
Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen This Caspase-8 antibody was raised against a synthetic peptide corresponding to amino acid residues 217-222 of human Caspase-8.
Full Name Caspase-8 antibody (mAb) (Clone 90A992)
Synonyms Caspase-8, Caspase8, Caspase 8, mAb, monoclonal, CASP8, apoptosis-related cysteine peptidase 8, antibody, antibodies
Molecular weight 55 kDa
GenBank accession numberNP_001073593
RRIDAB_2793445
Purification Method Affinity Purified
Buffer PBS containing 0.02% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

背景介绍

Caspase-8 (CASP8 or apoptosis-related cysteine peptidase 8) is the most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adaptor molecule FADD recruits it to either receptor. Caspase-8 cleaves and activates Caspase-3, Caspase-4, Caspase-6, Caspase-7, Caspase-9 and Caspase-10.