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DMAP1 antibody (pAb)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IP, WB

Cat No : 61675,61676 61675

Synonyms

DMAP1, DNA Methyltransferase 1 Associated Protein 1, MEAF2, DNMAP1, SWC4, DNMT1-Associated Protein 1, DNA Methyltransferase 1-Associated Protein 1, DNMTAP1, DNMT1 Associated Protein 1, EAF2, KIAA1425, HDAC2, transcription, transcriptional repression, transcriptional activation, DAXX, GR, NuA4 histone acetyltransferase complex, antibody, antibodies, polyclonal, pab, western blotting, wb, immunoprecipitation, ip, sample

验证数据展示

  • DMAP1 antibody (pAb) tested by Immunoprecipitation. 10 ul of DMAP1 antibody was used to immunoprecipitate DMAP1 from 500 ug of HeLa nuclear cell extract (lane 2). 10 ul of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the DMAP1 antibody at a dilution of 1:500.

    DMAP1 antibody (pAb) tested by Immunoprecipitation. 10 ul of DMAP1 antibody was used to immunoprecipitate DMAP1 from 500 ug of HeLa nuclear cell extract (lane 2). 10 ul of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the DMAP1 antibody at a dilution of 1:500.

  • DMAP1 antibody (pAb) tested by Western blot. Nuclear extract (30 ug) of Caco-2 (Lane 1) and HeLa cells (Lane 2) probed with DMAP1 antibody at a 1:500 dilution.

    DMAP1 antibody (pAb) tested by Western blot. Nuclear extract (30 ug) of Caco-2 (Lane 1) and HeLa cells (Lane 2) probed with DMAP1 antibody at a 1:500 dilution.

产品信息

Tested Applications IP, WB

Applications Validated by Active Motif: IP: 10 ul per IP WB*: 1:500 - 1:2,000 dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.
Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen This antibody was raised against a peptide within the C-terminal region of human DMAP1.
Full Name DMAP1 antibody (pAb)
Synonyms DMAP1, DNA Methyltransferase 1 Associated Protein 1, MEAF2, DNMAP1, SWC4, DNMT1-Associated Protein 1, DNA Methyltransferase 1-Associated Protein 1, DNMTAP1, DNMT1 Associated Protein 1, EAF2, KIAA1425, HDAC2, transcription, transcriptional repression, transcriptional activation, DAXX, GR, NuA4 histone acetyltransferase complex, antibody, antibodies, polyclonal, pab, western blotting, wb, immunoprecipitation, ip, sample
Molecular weight 60 kDa
GenBank accession numberNP_061973
RRIDAB_2793730
Purification Method Affinity Purified
Buffer Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

背景介绍

DMAP1 (DNA Methyltransferase 1 Associated Protein 1) is involved in transcription repression and activation. Its interaction with HDAC2 may provide a mechanism for histone deacetylation in heterochromatin following replication of DNA at late firing origins. Can also repress transcription independently of histone deacetylase activity. May specifically potentiate DAXX-mediated repression of glucocorticoid receptor-dependent transcription. Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Participates in the nuclear localization of URI1 and increases its transcriptional corepressor activity.