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DNMT1 antibody (pAb)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF, IP, WB

Cat No : 39905,39906 39905

Synonyms

DNMT1, DNMT-1, DNMT 1, DNA Methyltransferase 1, antibody, antibodies, DNA methylation, stem cell, stem cells, western blotting, wb, immunoprecipitation, ip, polyclonal, pab, sample

验证数据展示

  • DNMT1 antibody (pAb) tested by Immunoprecipitation. 10 ug of DNMT1 antibody was used to immunoprecipitate DNMT1 from 250 ug of HeLa nuclear cell extract (lane 2). 10 ug of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the DNMT1 antibody at a dilution of 0.5 ug/ml.

    DNMT1 antibody (pAb) tested by Immunoprecipitation. 10 ug of DNMT1 antibody was used to immunoprecipitate DNMT1 from 250 ug of HeLa nuclear cell extract (lane 2). 10 ug of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the DNMT1 antibody at a dilution of 0.5 ug/ml.

  • DNMT1 antibody (pAb) tested by Western blot. Nuclear extract of HeLa cells (20 ug per lane) probed with DNMT1 antibody (pAb) at a dilution of 0.5 ug/ml.

    DNMT1 antibody (pAb) tested by Western blot. Nuclear extract of HeLa cells (20 ug per lane) probed with DNMT1 antibody (pAb) at a dilution of 0.5 ug/ml.

  • Detection of DNMT1 by immunofluorescence. U2OS cells were stained with DNMT1 antibody at a dilution of 1:500. Left panel: DAPI. Middle panel: DNMT1 antibody staining. Right panel: merge.

    Detection of DNMT1 by immunofluorescence. U2OS cells were stained with DNMT1 antibody at a dilution of 1:500. Left panel: DAPI. Middle panel: DNMT1 antibody staining. Right panel: merge.

产品信息

Tested Applications IF, IP, WB

Applications Validated by Active Motif: IP: 10 ug per IP WB*: 0.5 - 2 ug/ml dilution IF: 1:500 dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot. For DNMT1, we also offer AbFlex DNMT1 Recombinant Antibody (rAb). For details, see Catalog No. 91175.
Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen This DNMT1 antibody was raised against a recombinant protein corresponding to amino acids 1-110 of human DNMT1.
Full Name DNMT1 antibody (pAb)
Synonyms DNMT1, DNMT-1, DNMT 1, DNA Methyltransferase 1, antibody, antibodies, DNA methylation, stem cell, stem cells, western blotting, wb, immunoprecipitation, ip, polyclonal, pab, sample
Molecular weight 185 kDa
GenBank accession numberNP_001370
RRIDAB_2793389
Purification Method Protein A Chromatography
Buffer Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

背景介绍

DNMT1 (DNA (cytosine-5)-methyltransferase) proteins are involved in DNA methylation in which a methyl group is added to a cytosine residue on DNA, commonly at the C5 position of a CpG dinucleotide. Three families of DNMTs have been identified: DNMT1, DNMT2, and DNMT3 (comprised of DNMT3A and DNMT3B). DNMT1 is the most abundant DNMT in somatic cells and shows a preference for methylating hemi-methylated DNA. It is considered to be a maintenance DNA methyltransferase that is important in the maintenance of specific patterns of methylation throughout cellular divisions. Methylation of mammalian DNA has long been recognized to play a major role in a number of cellular functions such as embryonic development, genetic imprinting, X chromosome inactivation and the control of gene expression. DNA methylation is generally associated with transcriptional repression.