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EuHMT1 antibody (pAb)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB

Cat No : 39777,39778 39777

Synonyms

Euchromatic Histone Methyltransferase 1, EHMT1, EuHMTase1, G9a like protein, GLP1, set domain, cell cycle, e2f, Myc, lys9, chromatin, kmt1d, sample

验证数据展示

  • EuHMT1 antibody (pAb) tested by Western blot. Nuclear extract of HeLa cells (20 ug) probed with EuHMT1 antibody (1 ug/ml dilution).

    EuHMT1 antibody (pAb) tested by Western blot. Nuclear extract of HeLa cells (20 ug) probed with EuHMT1 antibody (1 ug/ml dilution).

产品信息

Tested Applications WB

Applications Validated by Active Motif: WB*: 1 - 2 ug/ml dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.
Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen This EuHMT1 antibody was raised against a peptide derived from the N-terminal region of human EuHMT1.
Full Name EuHMT1 antibody (pAb)
Synonyms Euchromatic Histone Methyltransferase 1, EHMT1, EuHMTase1, G9a like protein, GLP1, set domain, cell cycle, e2f, Myc, lys9, chromatin, kmt1d, sample
Molecular weight 155 kDa
GenBank accession numberNP_079033
RRIDAB_2793339
Purification Method Protein A Chromatography
Buffer PBS pH 7.5 containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

背景介绍

EuHMT1 (Euchromatic Histone Methyltransferase 1, EHMT1, EuHMTase1, G9a like protein, GLP1) is a SET domain-containing histone methyltransferase specific for lysine 9 of histone H3. It is possible that this protein is involved in the silencing of Myc and E2F-responsive genes and thus could play a role in regulating cell cycle transitions from G0 to G1.