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FlexAble CoraLite® Plus 647 Antibody Labeling Kit for Rabbit IgG

Application

IF, FC, WB

Reactivity

Rabbit

Conjugate

CoraLite® Plus 647

Cat No : KFA003

Print Protocol

Validation Data Gallery

  • Immunofluorescence of rat brain tissue: FFPE rat brain tissue sections were stained with anti-MAP2 (17490-1-AP) labeled with FlexAble CoraLite® Plus 555 Kit (KFA002, orange), anti-GFAP (16825-1-AP) labeled with FlexAble CoraLite® Plus 647 Kit (KFA003, magenta) and CoraLite®488-conjugated TDP-43 antibody (CL488-10782, green).

    Immunofluorescence of rat brain tissue: FFPE rat brain tissue sections were stained with anti-MAP2 (17490-1-AP) labeled with FlexAble CoraLite® Plus 555 Kit (KFA002, orange), anti-GFAP (16825-1-AP) labeled with FlexAble CoraLite® Plus 647 Kit (KFA003, magenta) and CoraLite®488-conjugated TDP-43 antibody (CL488-10782, green).

  • Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-COXIV (11242-1-AP) labeled with FlexAble CoraLite® Plus 555 Kit (KFA002, yellow), anti-GM130 (11308-1-AP) labeled with FlexAble CoraLite® Plus 647 Kit (KFA003, magenta) and DAPI (blue).​ Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

    Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-COXIV (11242-1-AP) labeled with FlexAble CoraLite® Plus 555 Kit (KFA002, yellow), anti-GM130 (11308-1-AP) labeled with FlexAble CoraLite® Plus 647 Kit (KFA003, magenta) and DAPI (blue).​ Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

  • Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-TDP-43 (10782-2-AP) labeled with FlexAble CoraLite® 488 Kit (KFA001, green), anti-Tom20 (11802-1-AP) labeled with FlexAble CoraLite® Plus 555 Kit (KFA002, red) and anti-Lamin B1 (12987-1-AP) labeled with FlexAble CoraLite® Plus 647 Kit (KFA003, cyan).​ Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

    Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-TDP-43 (10782-2-AP) labeled with FlexAble CoraLite® 488 Kit (KFA001, green), anti-Tom20 (11802-1-AP) labeled with FlexAble CoraLite® Plus 555 Kit (KFA002, red) and anti-Lamin B1 (12987-1-AP) labeled with FlexAble CoraLite® Plus 647 Kit (KFA003, cyan).​ Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

  • Flow cytometry of PBMCs. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD45 labeled with FlexAble CoraLite® Plus 647 Kit (KFA003) and anti-CD3 (clone UCHT1, 65151-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA022).

    Flow cytometry of PBMCs. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD45 labeled with FlexAble CoraLite® Plus 647 Kit (KFA003) and anti-CD3 (clone UCHT1, 65151-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA022).

  • Flow cytometry of Jurkat cells. 1X10^6 Jurkat cells were stained with 0.5 µg anti-HSP90 antibody (13171-1-AP) labeled with FlexAble CoraLite® Plus 647 Kit (KFA003, cyan) or with isotype control antibody labeled with FlexAble CoraLite® Plus 647 Kit (KFA003, red).

    Flow cytometry of Jurkat cells. 1X10^6 Jurkat cells were stained with 0.5 µg anti-HSP90 antibody (13171-1-AP) labeled with FlexAble CoraLite® Plus 647 Kit (KFA003, cyan) or with isotype control antibody labeled with FlexAble CoraLite® Plus 647 Kit (KFA003, red).

  • WB of HeLa cell lysates: HeLa cell lysates were detected with anti-GAPDH (10494-1-AP) labeled with FlexAble CoraLite® Plus 647 Kit (KFA003, red) and anti-NUDT21 (66335-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA022, green).

    WB of HeLa cell lysates: HeLa cell lysates were detected with anti-GAPDH (10494-1-AP) labeled with FlexAble CoraLite® Plus 647 Kit (KFA003, red) and anti-NUDT21 (66335-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA022, green).

  • WB of HepG2 cell lysates: siRNA transfected HepG2 cell lysates were detected with anti-CCDC111 (29824-1-AP) labeled with FlexAble CoraLite® Plus 647 Kit (KFA003, red) and anti-PCNA (60097-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA022, green).

    WB of HepG2 cell lysates: siRNA transfected HepG2 cell lysates were detected with anti-CCDC111 (29824-1-AP) labeled with FlexAble CoraLite® Plus 647 Kit (KFA003, red) and anti-PCNA (60097-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA022, green).

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    FlexAble Workflow

产品介绍

FlexAble CoraLite® Plus 647 Antibody Labeling Kit for Rabbit IgG is a novel antibody labeling kit that uses an affinity linker to conjugate CoraLite® Plus 647 in any buffer condition to rabbit IgG primary antibodies from any supplier. One labeling reaction requires only 0.5µg of antibody – regardless of the antibody concentration. Label your rabbit IgG antibody in <10 min without any additional equipment.


Product name                

FlexAble CoraLite® Plus 647 Antibody Labeling Kit for Rabbit IgG                

Assay type                

Antibody labeling                

Tested applications                

IF, FC, WB                

Species Reactivity                

Rabbit IgG                

Antibody amount per labeling reaction          

0.5 µg antibody                

Conjugate                

CoraLite® Plus 647 

Excitation / Emission maxima wavelengths               

654 nm / 674 nm                


Component            10 rxns            50 rxns
           		4×50 rxns            
CoraLite® Plus 650 FlexLinker for Rabbit IgG10 μL50 μL4×50 μL
FlexQuencher for Rabbit IgG20 μL100 μL4×100 μL
FlexBuffer100 μL500 μL4×500 μL
Manual1 Copy
Protocol Magnet1 Piece


Storage Condition

Store for 1 year at -20°C or for 6 months at +4°C after shipment. Avoid exposure to light.