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HDAC1 antibody (pAb)

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

ChIP, ChIP-Seq, WB

Cat No : 40967 40967

Synonyms

HDAC1, HDAC-1, HDAC 1, pAb, polyclonal, Histone Deacetylase 1, antibody, antibodies, stem cell, stem cells, chiphsabs

验证数据展示

产品信息

Tested Applications ChIP, ChIP-Seq, WB

Applications Validated by Active Motif: ChIP: 4 ug per ChIP ChIP-Seq & ChIP-chip: 4 ug each WB: 1 - 2 ug/ml dilution For optimal results in Western blotting, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background. Individual optimization may be required. For HDAC1, we also offer AbFlex HDAC1 Recombinant Antibody (rAb). For details, see Catalog No. 91215.
Tested Reactivity Human, Mouse, Rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen This HDAC1 antibody was raised against a mixture of synthetic peptides corresponding to amino acid residues 1-5, 433-448 and 467-482 of human HDAC1.
Full Name HDAC1 antibody (pAb)
Synonyms HDAC1, HDAC-1, HDAC 1, pAb, polyclonal, Histone Deacetylase 1, antibody, antibodies, stem cell, stem cells, chiphsabs
Molecular weight 60 kDa
GenBank accession numberNP_004955
RRIDAB_2614948
Purification Method Affinity Purified
Buffer PBS containing 0.02% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

背景介绍

HDAC1 (Histone Deacetylase 1, also designated HD1) is a member of the class I mammalian histone deacetylases (HDACs) involved in regulating chromatin structure during transcription. These enzymes catalyze the removal of acetyl groups from lysine residues of histones and other cellular proteins. Lysine N-e-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in regulation of gene expression in various cellular functions. It consists of the transfer of an acetyl moiety from an acetyl coenzyme A to the e-amino group of a lysine residue. In vivo, acetylation is controlled by the antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs). The HDACs are grouped into four classes, on the basis of similarity to yeast counterparts: HDAC class I (HDAC1, HDAC2, HDAC3 and HDAC8), class II (HDAC4, HDAC5, HDAC6, HDAC7, HDAC9 and HDAC10), class III (SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6 and SIRT-7) and class IV (HDAC11). HDAC1 and HDAC2 are recruited to Mad-Max complexes, which associate with the mSin3 scaffold protein, and are required for the transcriptional repression of Mad-Max target genes. HDAC1 is also involved in the regulation of p53. HDAC1 is expressed in various tissues. HDAC1, HDAC2 and HDAC3 are also ubiquitously expressed and can deacetylate both Histone H3 and Histone H4 in free histones or nucleosome substrate.