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Histone H3K9ac antibody (mAb) (Clone 2G1F9)

Host / Isotype

Rat / IgG2a

Reactivity

Human, Wide Range Predicted

Applications

ChIP, ChIP-Seq, ICC, IF, WB

CloneNo.

2G1F9

Cat No : 61663,61664 61663

Synonyms

Histone H3K9ac, histone H3, histone, H3, histone-H3, histoneH3,H3 AcK9, Histone H3 Acetyl Lys9, Histone H3 Acetyl K9, Histone H3 Ac Lys9, H3 Acetyl Lys9, H3 Acetyl K9, H3 Ac Lys9, Histone H3 Acetylated Lysine 9, Histone H3 Acetyl Lysine9, H3 Acetylated Lysine 9, H3 Acetyl Lysine9, antibody, antibodies, H3K9Ac, Immunocytochemistry, ICC, Immunofluorescence, IF, western blotting, wb, monoclonal, mab, sample

验证数据展示

  • Histone H3K9ac antibody (mAb) (Clone 2G1F9) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with 15 ug of chromatin from a human medulloblastoma cell line and 4 ug of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 6 million sequence tags were mapped to identify Histone H3K9ac binding sites. The image shows binding across a region of chromosome 22. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here.

    Histone H3K9ac antibody (mAb) (Clone 2G1F9) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with 15 ug of chromatin from a human medulloblastoma cell line and 4 ug of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 6 million sequence tags were mapped to identify Histone H3K9ac binding sites. The image shows binding across a region of chromosome 22. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here.

  • Histone H3K9ac antibody (mAb) (Clone 2G1F9) tested by immunofluorescence. Left: HeLa cell stained with H3.1 / 3.2 antibody (mAb). Right: Hoechst.

    Histone H3K9ac antibody (mAb) (Clone 2G1F9) tested by immunofluorescence. Left: HeLa cell stained with H3.1 / 3.2 antibody (mAb). Right: Hoechst.

  • Histone H3K9ac antibody (mAb) (Clone 2G1F9) tested by Western blot. HeLa nuclear extract (20 ug per lane) probed with Histone H3K9ac antibody (mAb) (1 ug/ml). Lane 1: untreated cells. Lane 2: cells treated with sodium butyrate.

    Histone H3K9ac antibody (mAb) (Clone 2G1F9) tested by Western blot. HeLa nuclear extract (20 ug per lane) probed with Histone H3K9ac antibody (mAb) (1 ug/ml). Lane 1: untreated cells. Lane 2: cells treated with sodium butyrate.

产品信息

Tested Applications ChIP, ChIP-Seq, ICC, IF, WB

Applications Validated by Active Motif: ChIP: 5 - 10 ug per ChIP ChIP-Seq: 5 - 10 ug each ICC/IF: 1 - 2 ug/ml dilution WB*: 0.5 - 2 ul/ml dilution ChIP-Seq validation was performed by Active Motif's Epigenetics Services; the complete data set is available in the UCSC Genome Browser by clicking here. *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot. For Histone H3K9ac, we also offer AbFlex Histone H3K9ac Recombinant Antibody (rAb). For details, see Catalog No. 91103.
Tested Reactivity Human, Wide Range Predicted
Host / Isotype Rat / IgG2a
Class Monoclonal
Type Antibody
ModificationAcetylated
Immunogen This antibody was raised against a peptide containing acetyl-lysine 9 of human Histone H3.
Full Name Histone H3K9ac antibody (mAb) (Clone 2G1F9)
Synonyms Histone H3K9ac, histone H3, histone, H3, histone-H3, histoneH3,H3 AcK9, Histone H3 Acetyl Lys9, Histone H3 Acetyl K9, Histone H3 Ac Lys9, H3 Acetyl Lys9, H3 Acetyl K9, H3 Ac Lys9, Histone H3 Acetylated Lysine 9, Histone H3 Acetyl Lysine9, H3 Acetylated Lysine 9, H3 Acetyl Lysine9, antibody, antibodies, H3K9Ac, Immunocytochemistry, ICC, Immunofluorescence, IF, western blotting, wb, monoclonal, mab, sample
Molecular weight 17 kDa
GenBank accession numberNP_003522
RRIDAB_2793725
Purification Method Protein G Chromatography
Buffer Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

背景介绍

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. Lysine N-e-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Histone H3 Lys9 can also be mono-, di- or trimethylated. The methylation of this residue is often associated with transcriptional repression. However, acetylation of histone H3 Lys9 is associated with transcriptional activation of the genes.