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Nanog antibody (mAb) (Clone 2E6E1)

Host / Isotype

Rat / IgG2a

Reactivity

Mouse

Applications

ICC, IF, WB

CloneNo.

2E6E1

Cat No : 61627,61628 61627

Synonyms

Nanog, Nanog Homeobox, Homeobox Transcription Factor Nanog, hNanog, Homeobox Protein NANOG, Homeobox Transcription Factor Nanog-Delta 48, antibody, monoclonal, mAb, wb, western blotting, sample, immunofluorescence, immunocytochemistry, IF, ICC, transcription regulator, stem cells, embryonic stem cells, es, smad1, activator, repressor, sacrococcygeal teratoma, ovarian germ cell tumor

验证数据展示

  • Nanog antibody (mAb) (Clone 2E6E1) tested by immunofluorescence. Top: HeLa cells stained with Nanog antibody (mAb). Bottom: Hoechst staining.

    Nanog antibody (mAb) (Clone 2E6E1) tested by immunofluorescence. Top: HeLa cells stained with Nanog antibody (mAb). Bottom: Hoechst staining.

  • Nanog antibody (mAb) (Clone 2E6E1) tested by Western blot. Nanog antibody detection by Western blot. The analysis was performed using 30 ug of mouse ES nuclear extract and Nanog antibody at a 2 ug/ml dilution.

    Nanog antibody (mAb) (Clone 2E6E1) tested by Western blot. Nanog antibody detection by Western blot. The analysis was performed using 30 ug of mouse ES nuclear extract and Nanog antibody at a 2 ug/ml dilution.

产品信息

Tested Applications ICC, IF, WB

Applications Validated by Active Motif: ICC/IF: 2 - 10 ug/ml dilution WB*: 1 - 2 ug/ml dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot. This antibody is also available as an AbFlex engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody, see Catalog No. 91251.
Tested Reactivity Mouse
Host / Isotype Rat / IgG2a
Class Monoclonal
Type Antibody
Immunogen This antibody was raised against a peptide comprising amino acids 2-25 of mouse Nanog.
Full Name Nanog antibody (mAb) (Clone 2E6E1)
Synonyms Nanog, Nanog Homeobox, Homeobox Transcription Factor Nanog, hNanog, Homeobox Protein NANOG, Homeobox Transcription Factor Nanog-Delta 48, antibody, monoclonal, mAb, wb, western blotting, sample, immunofluorescence, immunocytochemistry, IF, ICC, transcription regulator, stem cells, embryonic stem cells, es, smad1, activator, repressor, sacrococcygeal teratoma, ovarian germ cell tumor
Molecular weight 45 kDa
GenBank accession numberNP_001276757
RRIDAB_2793709
Purification Method Protein G Chromatography
Buffer Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

背景介绍

Nanog (Nanog homeobox) is a transcriptional regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation (By similarity).