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OGA/O-GlcNAcase antibody (pAb)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IP, WB

Cat No : 61425,61426 61425

Synonyms

OGA/O-GlcNAcase, meningioma expressed antigen 5 (hyaluronidase), O-GlcNAcase, MEA5, bifunctional protein NCOAT, NCOAT, hyaluronidase in meningioma, OGA, Meningioma-expressed antigen 5, Nuclear cytoplasmic O-GlcNAcase and acetyltransferase, antibody, polyclonal, pAb, western blotting, wb, immunoprcipitation, ip, sample

验证数据展示

  • OGA/O-GlcNAcase antibody (pAb) tested by Immunoprecipitation. 10 ul of OGA/O-GlcNAcase antibody was used to immunoprecipitate OGA/O-GlcNAcase from 400 ug of HeLa whole cell extract (lane 2). 10 ul of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the OGA/O-GlcNAcase antibody at a dilution of 1:1,000.

    OGA/O-GlcNAcase antibody (pAb) tested by Immunoprecipitation. 10 ul of OGA/O-GlcNAcase antibody was used to immunoprecipitate OGA/O-GlcNAcase from 400 ug of HeLa whole cell extract (lane 2). 10 ul of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the OGA/O-GlcNAcase antibody at a dilution of 1:1,000.

  • OGA/O-GlcNAcase pAb tested by Western blot. OGA/O-GlcNAcase detection by Western blot. The analysis was performed using 20 ug HeLa whole cell extract and OGA/O-GlcNAcase at a 1:1,000 dilution.

    OGA/O-GlcNAcase pAb tested by Western blot. OGA/O-GlcNAcase detection by Western blot. The analysis was performed using 20 ug HeLa whole cell extract and OGA/O-GlcNAcase at a 1:1,000 dilution.

产品信息

Tested Applications IP, WB

Applications Validated by Active Motif: IP: 10 ul per IP WB: 1:500 - 1:2,000 dilution The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.
Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen This antibody was raised against a peptide within the N-terminal region of human OGA/ O-GlcNAcase.
Full Name OGA/O-GlcNAcase antibody (pAb)
Synonyms OGA/O-GlcNAcase, meningioma expressed antigen 5 (hyaluronidase), O-GlcNAcase, MEA5, bifunctional protein NCOAT, NCOAT, hyaluronidase in meningioma, OGA, Meningioma-expressed antigen 5, Nuclear cytoplasmic O-GlcNAcase and acetyltransferase, antibody, polyclonal, pAb, western blotting, wb, immunoprcipitation, ip, sample
Molecular weight 115 kDa
GenBank accession numberNP_036347
RRIDAB_2793630
Purification Method Affinity Purified
Buffer Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

背景介绍

OGA / O GlcNAcase (O-linked N-acetylglucosamine (GlcNAc) -ase) is a glycosidase that removes O-linked N-acetylglucosamine (O-GlcNAc) modifications from serine and threonine residues on nuclear and cytoplasmic proteins. This dynamic modification is catalyzed by OGT and plays a role in homeostatic mechanisms and influences gene expression.