• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

SMARCB1 antibody (mAb) (Clone 2C2)

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

ICC, IF, WB

CloneNo.

2C2

Cat No : 61649,61650 61649

Synonyms

SMARCB1, SWI/SNF Related, Matrix Associated Actin Dependent Regulator Of Chromatin, Subfamily B Member 1, SNF5, SNF5L1, SWNTS1, INI1, Homolog-Like 1, Malignant Rhabdoid Tumor Suppressor, Integrase Interactor 1, Integrase Interactor 1 Protein, Sucrose Nonfermenting, BAF47, yeast, BRG1-Associated Factor 47, Sfh1p, hSNF5, Snr1, SNF5 Homolog, hSNFS, MRD15, Sucrose Nonfermenting Yeast, Homolog-Like 1, RDT, SWI/SNF-Related Matrix-Associated Actin-Dependent Regulator Of Chromatin Subfamily B Member 1, RTPS1, SWI/SNF-Related Matrix-Associated Protein, monoclonal, mab, immunofluorescence, if, immunocytochemistry, icc, western blotting, wb, antibody, antibodies, sample

验证数据展示

  • SMARCB1 antibody (mAb) (Clone 2C2) tested by immunofluorescence. Top: HeLa cells stained with SMARCB1 antibody (mAb). Bottom: Hoechst staining.

    SMARCB1 antibody (mAb) (Clone 2C2) tested by immunofluorescence. Top: HeLa cells stained with SMARCB1 antibody (mAb). Bottom: Hoechst staining.

  • SMARCB1 antibody (mAb) (Clone 2C2) tested by Western blot. SMARCB1 antibody detection by Western blot. The analysis was performed using 20 ug of Jurkat nuclear extract and SMARCB1 antibody at a 2 ug/ml dilution.

    SMARCB1 antibody (mAb) (Clone 2C2) tested by Western blot. SMARCB1 antibody detection by Western blot. The analysis was performed using 20 ug of Jurkat nuclear extract and SMARCB1 antibody at a 2 ug/ml dilution.

产品信息

Tested Applications ICC, IF, WB

Applications Validated by Active Motif: ICC/IF: 2 - 10 ug/ml dilution WB*: 0.5 - 2 ug/ml dilution The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required. *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.
Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen This antibody was raised against full-length recombinant human SMARCB1 protein.
Full Name SMARCB1 antibody (mAb) (Clone 2C2)
Synonyms SMARCB1, SWI/SNF Related, Matrix Associated Actin Dependent Regulator Of Chromatin, Subfamily B Member 1, SNF5, SNF5L1, SWNTS1, INI1, Homolog-Like 1, Malignant Rhabdoid Tumor Suppressor, Integrase Interactor 1, Integrase Interactor 1 Protein, Sucrose Nonfermenting, BAF47, yeast, BRG1-Associated Factor 47, Sfh1p, hSNF5, Snr1, SNF5 Homolog, hSNFS, MRD15, Sucrose Nonfermenting Yeast, Homolog-Like 1, RDT, SWI/SNF-Related Matrix-Associated Actin-Dependent Regulator Of Chromatin Subfamily B Member 1, RTPS1, SWI/SNF-Related Matrix-Associated Protein, monoclonal, mab, immunofluorescence, if, immunocytochemistry, icc, western blotting, wb, antibody, antibodies, sample
Molecular weight 45 kDa
GenBank accession numberNP_001007469
RRIDAB_2793719
Purification Method Protein A Chromatography
Buffer Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

背景介绍

SMARCB1 (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator Of Chromatin, Subfamily B, Member 1) is a core component of the BAF (hSWI/SNF) complex. This ATP-dependent chromatin-remodeling complex plays important roles in cell proliferation and differentiation, in cellular antiviral activities and inhibition of tumor formation. The BAF complex is able to create a stable, altered form of chromatin that constrains fewer negative supercoils than normal. This change in supercoiling would be due to the conversion of up to one-half of the nucleosomes on polynucleosomal arrays into asymmetric structures, termed altosomes, each composed of 2 histones octamers. Stimulates in vitro the remodeling activity of SMARCA4/BRG1/BAF190A. Involved in activation of CSF1 promoter. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity). Plays a key role in cell-cycle control and causes cell cycle arrest in G0/G1. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.