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Tet3 antibody (mAb) (Clone 23B9)

Host / Isotype

Rat / IgG2a

Reactivity

Mouse

Applications

ICC, IF, IP, WB

CloneNo.

23B9

Cat No : 61743,61744 61743

Synonyms

Tet Methylcytosine Dioxygenase 3, Tet Oncogene Family Member 3, EC 1.14.11.n2, KIAA0401, Probable Methylcytosine Dioxygenase TET3, Putative Methylcytosine Dioxygenase, Methylcytosine Dioxygenase TET3, HCG_40738, antibody, antibodies, monoclonal, mab, if, icc, ip, wb, immunofluorescence, immunocytochemistry, immunoprecipitation, western blotting, sample

验证数据展示

产品信息

Tested Applications ICC, IF, IP, WB

Tested Reactivity Mouse
Host / Isotype Rat / IgG2a
Class Monoclonal
Type Antibody
Immunogen This antibody was raised against a recombinant protein corresponding amino acids 976-1521 of mouse Tet3.
Full Name Tet3 antibody (mAb) (Clone 23B9)
Synonyms Tet Methylcytosine Dioxygenase 3, Tet Oncogene Family Member 3, EC 1.14.11.n2, KIAA0401, Probable Methylcytosine Dioxygenase TET3, Putative Methylcytosine Dioxygenase, Methylcytosine Dioxygenase TET3, HCG_40738, antibody, antibodies, monoclonal, mab, if, icc, ip, wb, immunofluorescence, immunocytochemistry, immunoprecipitation, western blotting, sample
Molecular weight 180 kDa
GenBank accession numberNP_898961
RRIDAB_2793753
Purification Method Protein G Chromatography
Buffer Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

背景介绍

Tet3 (Tet Methylcytosine Dioxygenase 3) is a dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and plays a key role in epigenetic chromatin reprogramming in the zygote following fertilization. Also mediates subsequent conversion of 5hmC into 5-formylcytosine (5fC), and conversion of 5fC to 5-carboxylcytosine (5caC). Conversion of 5mC into 5hmC, 5fC and 5caC probably constitutes the first step in cytosine demethylation. In zygotes, DNA demethylation occurs selectively in the paternal pronucleus before the first cell division, while the adjacent maternal pronucleus and certain paternally-imprinted loci are protected from this process. Participates in DNA demethylation in the paternal pronucleus by mediating conversion of 5mC into 5hmC, 5fC and 5caC. Does not mediate DNA demethylation of maternal pronucleus because of the presence of DPPA3/PGC7 on maternal chromatin that prevents TET3-binding to chromatin (By similarity). In addition to its role in DNA demethylation, also involved in the recruitment of the O-GlcNAc transferase OGT to CpG-rich transcription start sites of active genes, thereby promoting histone H2B GlcNAcylation by OGT.