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验证数据展示

  • WB analysis of human brain using 11159-1-AP

    WB analysis of human brain using 11159-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 11159-1-AP (ABCD1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse liver using 11159-1-AP

    IP experiment of mouse liver using 11159-1-AP

    IP result of anti-ABCD1 (IP:11159-1-AP, 4ug; Detection:11159-1-AP 1:500) with mouse liver tissue lysate 5000ug.

  • IHC staining of human lung cancer using 11159-1-AP

    IHC staining of human lung cancer using 11159-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 11159-1-AP (ABCD1 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with ABCD1 antibody included
  • IF Staining of HeLa using 11159-1-AP

    IF Staining of HeLa using 11159-1-AP

    Immunofluorescent analysis of HeLa cells using 11159-1-AP (ABCD1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

ABCD1 Polyclonal antibody

ABCD1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 11159-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

别名

EC:3.1.2.-, ATP-binding cassette sub-family D member 1, ATP binding cassette sub family D member 1, ALD, ABCD 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman brain tissue, HeLa cells, mouse liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ABCD1 Ready-To-Use IHC Kit. ABCD1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11159-1-AP targets ABCD1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 ABCD1 fusion protein Ag1635 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ATP-binding cassette, sub-family D (ALD), member 1
别名 EC:3.1.2.-, ATP-binding cassette sub-family D member 1, ATP binding cassette sub family D member 1, ALD, ABCD 1
计算分子量 745 aa, 83 kDa
观测分子量 75 kDa
GenBank蛋白编号BC025358
基因名称 ABCD1
Gene ID (NCBI) 215
RRIDAB_2219775
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP33897
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ABCD1 (also known as ALDP) is a member of the ATP-binding cassette (ABC) transporter superfamily which functions as transporter for a wide variety of substrates. It localizes to the peroxisomal membrane. The exact function is not clear so far. Various mutations of ABCD1 cause X-linked adrenoleukodystrophy (X-ALD), an inherited neurodegenerative disease affecting the nervous system white matter and adrenal cortex.

实验方案

Product Specific Protocols
WB protocol for ABCD1 antibody 11159-1-APDownload protocol
IHC protocol for ABCD1 antibody 11159-1-APDownload protocol
IF protocol for ABCD1 antibody 11159-1-APDownload protocol
IP protocol for ABCD1 antibody 11159-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cancers (Basel)

Prostate Cancer Proliferation Is Affected by the Subcellular Localization of MCT2 and Accompanied by Significant Peroxisomal Alterations.

Authors - Isabel Valença
View Article
mouseIF

J Biomed Res

Germ cell-specific deletion of Pex3 reveals essential roles of PEX3-dependent peroxisomes in spermiogenesis

Authors - Yejin Yao
View Article