验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17693-1-AP targets ABL2 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, CoIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | ABL2 fusion protein Ag11860 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | v-abl Abelson murine leukemia viral oncogene homolog 2 (arg, Abelson-related gene) |
| 别名 | Tyrosine-protein kinase ABL2, EC:2.7.10.2, ARG, ABLL, Abelson-related gene protein |
| 计算分子量 | 1167 aa, 127 kDa |
| 观测分子量 | 128-140 kDa |
| GenBank蛋白编号 | BC065912 |
| 基因名称 | ABL2 |
| Gene ID (NCBI) | 27 |
| RRID | AB_2289025 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P42684 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ABL2(Abelson tyrosine-protein kinase 2) is also named as ABLL, ARG and belongs to the protein kinase superfamily. It is a membrane-anchored nonreceptor tyrosine kinase that is a novel mediator of SEMA3F-induced RhoA inactivation and collapsing activity and it has a role in cytoskeletal rearrangements by its C-terminal F-actin- and microtubule-binding sequences. ABL2 can be polyubiquitinated and the polyubiquitination of ABL2 leads to degradation. This protein has 9 isoforms produced by alternative splicing with the molecular weight from 114 kDa to 128 kDa. The antibody is specific to the others except the 60 kDa isoform of ABL2.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ABL2 antibody 17693-1-AP | Download protocol |
| IHC protocol for ABL2 antibody 17693-1-AP | Download protocol |
| IP protocol for ABL2 antibody 17693-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Bioact Mater High proportion strontium-doped micro-arc oxidation coatings enhance early osseointegration of titanium in osteoporosis by anti-oxidative stress pathway. | ||
Theranostics Non-invasive photobiomodulation treatment in an Alzheimer Disease-like transgenic rat model. | ||
Elife Abelson tyrosine-protein kinase 2 regulates myoblast proliferation and controls muscle fiber length.
| ||
Oncogene Phosphorylation of MICAL2 by ARG promotes head and neck cancer tumorigenesis by regulating skeletal rearrangement | ||
Genomics Bone marrow mesenchymal stem cells-derived exosomal microRNA-19b-1-5p reduces proliferation and raises apoptosis of bladder cancer cells via targeting ABL2. | ||
Biochim Biophys Acta Abl2 kinase phosphorylates Bi-organellar regulator MNRR1 in mitochondria, stimulating respiration. |
