ACAA1 Polyclonal antibody

ACAA1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 12319-2-AP

Print datasheet

Synonyms

Acetyl-CoA C-myristoyltransferase, Acetyl-CoA acyltransferase, Acetyl CoA acyltransferase, ACAA, 3-ketoacyl-CoA thiolase, peroxisomal



经过测试的应用

Positive WB detected inL02 cells, A549 cells, human lung tissue
Positive IHC detected inhuman thyroid cancer tissue, human lung tissue, human prostate cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU-251 cells
Planning an IHC experiment? We recommend our IHCeasy ACAA1 Ready-To-Use IHC Kit. ACAA1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12319-2-AP targets ACAA1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen ACAA1 fusion protein Ag2972 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name acetyl-Coenzyme A acyltransferase 1
Synonyms Acetyl-CoA C-myristoyltransferase, Acetyl-CoA acyltransferase, Acetyl CoA acyltransferase, ACAA, 3-ketoacyl-CoA thiolase, peroxisomal
Calculated Molecular Weight 424 aa, 44 kDa
Observed Molecular Weight 41 kDa
GenBank Accession NumberBC011977
Gene Symbol ACAA1
Gene ID (NCBI) 30
RRIDAB_2289045
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP09110
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACAA1 (acetyl-CoA acyltransferase 1) is an enzyme involved in lipid β-oxidation and provides substrates to the tricarboxylic acid (TCA) cycle, a critical step in cellular metabolism (PMID:33194642). ACAA1 is also a biomarker in type 2 diabetes (T2D), predicting the pre-diabetic metabolic signature in mouse models (PMID:25784953). Moreover, ACAA1 is highly expressed in TNBC, serving as a potential therapeutic target in ACAA1-high tumors and a predictive biomarker of resistance to CDK4/6 inhibitors for RB1-proficient patients (PMID:37129951).

实验方案

Product Specific Protocols
WB protocol for ACAA1 antibody 12319-2-APDownload protocol
IHC protocol for ACAA1 antibody 12319-2-APDownload protocol
IF protocol for ACAA1 antibody 12319-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Hum Mol Genet

Functional analysis of PEX13 mutation in a Zellweger syndrome spectrum patient reveals novel homooligomerization of PEX13 and its role in human peroxisome biogenesis.

Authors - Krause Cindy C
humanWB

Cell Mol Life Sci

Murine deficiency of peroxisomal L-bifunctional protein (EHHADH) causes medium-chain 3-hydroxydicarboxylic aciduria and perturbs hepatic cholesterol homeostasis.

Authors - Pablo Ranea-Robles
ratWB

EBioMedicine

PPARγ maintains the metabolic heterogeneity and homeostasis of renal tubules.

Authors - Zhongshi Lyu
mouseWB

Commun Biol

Transmembrane protein 135 regulates lipid homeostasis through its role in peroxisomal DHA metabolism

Authors - Michael Landowski
IF

J Agric Food Chem

Proteomic Analysis of Lysosomal Membrane Proteins in Bovine Mammary Epithelial Cells Illuminates Potential Novel Lysosome Functions in Lactation.

Authors - Chaochao Luo
humanIF

Sci Rep

Super-resolution imaging reveals the sub-diffraction phenotype of Zellweger Syndrome ghosts and wild-type peroxisomes.

Authors - Kareem Soliman