验证数据展示
经过测试的应用
| Positive WB detected in | Caco-2 cells, Jurkat cells, mouse kidney tissue, RAW 264.7 cells |
| Positive IP detected in | mouse kidney tissue |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17138-1-AP targets ACSS1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IP, IHC, ELISA Application Description |
| Cited Applications | WB, IHC, IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig |
| Immunogen | ACSS1 fusion protein Ag10896 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | acyl-CoA synthetase short-chain family member 1 |
| Synonyms | ACAS2L, ACECS1, AceCS2L, Acetyl coenzyme A synthetase 2 like, mitochondrial, ACSS1, KIAA1846 |
| Calculated Molecular Weight | 689 aa, 75 kDa |
| Observed Molecular Weight | 70-75 kDa |
| GenBank Accession Number | BC039261 |
| Gene Symbol | ACSS1 |
| Gene ID (NCBI) | 84532 |
| RRID | AB_2289182 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NUB1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The ACSS (acetyl-CoA synthetase) enzyme is the sole known mammalian enzyme that can catalyze the conversion of free acetate into acetyl coenzyme A (acetyl-CoA). The three known isoforms of human ACSS are termed ACSS1, ACSS2, and ACSS3. The main substrate of ACSS1 and ACSS2 is acetate, while the preferential substrate of ACSS3 is propionate. Two acetate related enzymes, ACSS1(GeneID: 84532) and ACSS2 (GeneID:55902) difer in their tissue distribution and subcellular localization. On the one hand, as a mitochondrial matrix enzyme, ACSS1 is expressed mainly in cardiac and skeletal muscle as well as brown adipose tissue. On the other hand, as a nuclear and cytoplasmic enzyme, ACSS2 is strongly expressed in the liver, kidney and heart and moderately expressed in the brain and testis.ACSS2 participates in lipid synthesis and facilitates protein acetylation by generating acetyl-CoA, while ACSS1 is involved in acetate oxidation. The functional diferences in these enzymes involve energy production through the tricarboxylic acid (TCA) cycle. Due to its more thorough utilization of intracellular acetate, ACSS2 is expressed in almost all cell types under diferent physiological conditions.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ACSS1 antibody 17138-1-AP | Download protocol |
| IHC protocol for ACSS1 antibody 17138-1-AP | Download protocol |
| IP protocol for ACSS1 antibody 17138-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Metab Acetate enables metabolic fitness and cognitive performance during sleep disruption | ||
EMBO J Sex-specific, reciprocal regulation of ERα and miR-22 controls muscle lipid metabolism in male mice. | ||
J Cell Physiol Acetyl-CoA synthases are essential for maintaining histone acetylation under metabolic stress during zygotic genome activation in pigs.
| ||
PLoS Comput Biol Inferring latent temporal progression and regulatory networks from cross-sectional transcriptomic data of cancer samples. | ||
Front Pharmacol Integrated Metabolomics and Network Pharmacology Approach to Explain Possible Action Mechanisms of Xin-Sheng-Hua Granule for Treating Anemia. |
