- Featured Product
- KD/KO Validated
smooth muscle actin Polyclonal antibody
smooth muscle actin Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (6)
Applications
FC, IF, IHC, IP, WB, ELISA and More (2)
Conjugate
Unconjugated
939
验证数据展示
经过测试的应用
Positive WB detected in | mouse colon tissue, HUVEC cells, human placenta tissue, A549 cells, mouse liver tissue, mouse kidney tissue, rat colon tissue, mouse heart tissue, rat heart tissue |
Positive IP detected in | mouse liver tissue |
Positive IHC detected in | human liver cancer tissue, human heart tissue, stromal tumor tissue, human hysteromyoma tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | C2C12 cells, H9C2 cells, mouse heart tissue, mouse small intestine tissue |
Positive FC detected in | C2C12 cell |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:1500-1:6000 |
Immunofluorescence (IF) | IF : 1:800-1:3200 |
Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
14395-1-AP targets smooth muscle actin in WB, IP, IHC, IF, FC, competitive binding assay, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | FC, IF, IHC, IP, WB, ELISA |
Cited Applications | CoIP, competitive binding assay, FC, IF, IHC, WB |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, sheep, monkey, rabbit, zebrafish, pig, canine |
Immunogen | smooth muscle actin fusion protein Ag5886 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | actin, alpha 2, smooth muscle, aorta |
Synonyms | a SMA, AAT6, ACTA2, ACTA2 Smooth muscle Actin, Actin, aortic smooth muscle, Actin, aortic smooth muscle Actin, ACTSA, ACTVS, Alpha actin 2, Alpha SMA, a-SMA, sma, smooth muscle actin, α SMA, α-sma |
Calculated Molecular Weight | 377 aa, 42 kDa |
Observed Molecular Weight | 43 kDa |
GenBank Accession Number | BC017554 |
Gene Symbol | ACTA2 |
Gene ID (NCBI) | 59 |
RRID | AB_2223009 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P62736 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for smooth muscle actin antibody 14395-1-AP | Download protocol |
IHC protocol for smooth muscle actin antibody 14395-1-AP | Download protocol |
IF protocol for smooth muscle actin antibody 14395-1-AP | Download protocol |
IP protocol for smooth muscle actin antibody 14395-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Metab Augmentation of scleral glycolysis promotes myopia through histone lactylation | ||
Mil Med Res Caveolin-1 is critical for hepatic iron storage capacity in the development of nonalcoholic fatty liver disease | ||
Nat Cell Biol LncGata6 maintains stemness of intestinal stem cells and promotes intestinal tumorigenesis. | ||
ACS Nano Endoscopy Deliverable and Mushroom-Cap-Inspired Hyperboloid-Shaped Drug-Laden Bioadhesive Hydrogel for Stomach Perforation Repair | ||
Nat Cell Biol Single-cell reconstruction of the adult human heart during heart failure and recovery reveals the cellular landscape underlying cardiac function. |