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  • Featured Product
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CoraLite®594-conjugated ALDH1A1 Monoclonal antibody

ALDH1A1 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1A10A2

Cat no : CL594-60171

Print datasheet

Synonyms

ALDC, ALDH E1, ALDH1, ALDH11, ALDH1A1, ALHDII, PUMB1, RALDH 1, RALDH1, Retinal dehydrogenase 1

验证数据展示

  • IF Staining of HeLa using CL594-60171

    IF Staining of HeLa using CL594-60171

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 ALDH1A1 antibody (CL594-60171, Clone: 1A10A2 ) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ALDH1A1 Ready-To-Use IHC Kit. ALDH1A1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60171-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-60171 targets ALDH1A1 in IF/ICC applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen ALDH1A1 fusion protein Ag8551 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name aldehyde dehydrogenase 1 family, member A1
Synonyms ALDC, ALDH E1, ALDH1, ALDH11, ALDH1A1, ALHDII, PUMB1, RALDH 1, RALDH1, Retinal dehydrogenase 1
Calculated Molecular Weight 501 aa, 55 kDa
GenBank Accession NumberBC001505
Gene Symbol ALDH1A1
Gene ID (NCBI) 216
RRIDAB_2883431
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP00352
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ALDH1A1(Aldehyde dehydrogenase family 1 member A1 ), also named as ALDC, ALDH1 and PUMB1, belongs to the aldehyde dehydrogenase family. The ALDH1A1 gene encodes a liver cytosolic isoform of acetaldehyde dehydrogenase, an enzyme involved in the major pathway of alcohol metabolism after alcohol dehydrogenase. ALDH1A1 plays a critical role in protection against oxidative stress-induced cytotoxicity in lens epithelial cells(PMID:19296407). And it is important for multiple biological activities including drug resistance, cell differentiation, and oxidative stress response(PMID:19025616). As a novel cancer stem cell marker, ALDH1A1 can be used for tumors whose corresponding normal tissues express ALDH1A1 in relatively restricted or limited levels such as breast, lung, ovarian or colon cancer(PMID: 20422001). This antibody is CL594(Ex/Em 593 nm/614 nm) conjugated.

实验方案

Product Specific Protocols
IF protocol for CL594 ALDH1A1 antibody CL594-60171Download protocol
Standard Protocols
Click here to view our Standard Protocols