ALPK1 Polyclonal antibody
ALPK1 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | COLO 320 cells, HEK-293 cells, HepG2 cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
The immunogen of 19107-1-AP is ALPK1 Fusion Protein expressed in E. coli.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IF, IHC |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Immunogen | ALPK1 fusion protein Ag5320 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | alpha-kinase 1 |
| Synonyms | 8430410J10Rik, alpha kinase 1, Alpha protein kinase 1, ALPK, ALPK1, Chromosome 4 kinase, KIAA1527, LAK |
| Calculated Molecular Weight | 139 kDa |
| Observed Molecular Weight | 140 kDa |
| GenBank Accession Number | BC060780 |
| Gene Symbol | ALPK1 |
| Gene ID (NCBI) | 80216 |
| RRID | AB_10638642 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96QP1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Alpha-protein kinase 1, also known as alpha-kinase 1 (ALPK1), is associated with chronic kidney disease (CKD), myocardial infarction, gout and type 2 diabetes mellitus (DM). ALPK1 mutant has been reported to cause severe defects in motor co‐ordination in mice. ALPK1 enhances the expression of proinflammatory cytokines including IL-1β, IL-8 and TGF-β1 in cultured human THP1 and human embryonic kidney 293 (HEK293) cells (PMID: 31557402, PMID: 26275947). ALPK1 short isoform (108 kDa) was highly expressed in brain, spinal cord, heart, lung, spleen, thymus, small intestine, skin and testis, while detectable inskeletal muscle and kidney. ALPK1 large isoform (130 kDa) appeared in heart, lung, thymus and skin (PMID: 21208416).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ALPK1 antibody 19107-1-AP | Download protocol |
| IHC protocol for ALPK1 antibody 19107-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Immunol Alpk1 Sensitizes Pancreatic Beta Cells to Cytokine-Induced Apoptosis via Upregulating TNF-α Signaling Pathway. | ||
J Bone Miner Res ALPK1 Accelerates the Pathogenesis of Osteoarthritis by Activating NLRP3 Signaling | ||
Int Immunopharmacol Neuroprotective effect of alpha-kinase 1 knockdown against cerebral ischemia through inhibition of the NF-κB pathway and neuroinflammation | ||
Mol Neurobiol ALPK1 Expressed in IB4-Positive Neurons of Mice Trigeminal Ganglions Promotes MIA-Induced TMJ pain | ||
Int Immunopharmacol Pyroptosis of chondrocytes activated by synovial inflammation accelerates TMJ osteoarthritis cartilage degeneration via ROS/NLRP3 signaling |