验证数据展示
经过测试的应用
| Positive WB detected in | A375 cells, mouse liver tissue |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
11964-1-AP targets ANGPTL3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, rat |
| Immunogen | ANGPTL3 fusion protein Ag2573 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | angiopoietin-like 3 |
| Synonyms | ANG 5, Angiopoietin 5, angiopoietin like 3, Angiopoietin like protein 3, Angiopoietin related protein 3, ANGPT5, ANGPTL3 |
| Calculated Molecular Weight | 460 aa, 54 kDa |
| Observed Molecular Weight | 54 kDa |
| GenBank Accession Number | BC058287 |
| Gene Symbol | ANGPTL3 |
| Gene ID (NCBI) | 27329 |
| RRID | AB_874076 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y5C1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ANGPTL3 belongs to the angiopoietin-like protein family. ANGPTL3 is mainly synthesized by liver cells and is notably expressed in kidney podocytes. Accumulating evidences have revealed that ANGPTL3 plays a critical role in both biological processes, such as lipid metabolism, angiogenesis and haematopoietic function and pathological changes, including atherosclerosis, carcinogenesis, nephrotic syndrome, diabetes, liver diseases and so on. Thus, ANGPTL3 may serve as a potential biomarker in these diseases.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ANGPTL3 antibody 11964-1-AP | Download protocol |
| IHC protocol for ANGPTL3 antibody 11964-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Sci Screening of novel serum biomarkers for gastric cancer in coastal populations using a protein microarray |
