验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, human brain tissue, mouse brain tissue, mouse thymus tissue, Raji cells, HeLa cells, MCF-7 cells, HEK-293T cells |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells, A2780 cells |
Planning an IHC experiment? We recommend our IHCeasy ANP32A Ready-To-Use IHC Kit. ANP32A primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 2 publications below |
| WB | See 3 publications below |
产品信息
15810-1-AP targets ANP32A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, chicken |
| Immunogen | ANP32A fusion protein Ag8546 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | acidic (leucine-rich) nuclear phosphoprotein 32 family, member A |
| Synonyms | MAPM, Leucine-rich acidic nuclear protein, LANP, I1PP2A, C15orf1 |
| Calculated Molecular Weight | 249 aa, 29 kDa |
| Observed Molecular Weight | 29 kDa |
| GenBank Accession Number | BC007200 |
| Gene Symbol | ANP32A |
| Gene ID (NCBI) | 8125 |
| RRID | AB_2056302 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P39687 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ANP32A, an acidic leucine-rich nuclear phosphoprotein, is a member of the inhibitor of the histone acetyltransferase (INHAT) complex and preferentially binds to unmodified histone H3 tails in vitro. ANP32A is expressed in normal tissues as well as in pancreatic, breast and prostate cancer and other malignant tumors. ANP32A plays an important role in cell proliferation, apoptosis, transcriptional regulation, signal transduction and other processes.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ANP32A antibody 15810-1-AP | Download protocol |
| IHC protocol for ANP32A antibody 15810-1-AP | Download protocol |
| IF protocol for ANP32A antibody 15810-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Adv Avian ANP32A incorporated in avian influenza A virions promotes interspecies transmission by priming early viral replication in mammals | ||
Cancer Cell Int Super enhancer related gene ANP32B promotes the proliferation of acute myeloid leukemia by enhancing MYC through histone acetylation
| ||
Arch Virol The interaction of cellular protein ANP32A with influenza A virus polymerase component PB2 promotes vRNA synthesis.
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