验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2400 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 5 publications below |
产品信息
16932-1-AP targets AP1B1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IF/ICC, IP, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Immunogen | AP1B1 fusion protein Ag10417 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | adaptor-related protein complex 1, beta 1 subunit |
| Synonyms | ADTB1, AP 1 complex subunit beta 1, AP105A, AP1B1, BAM22, Beta 1 adaptin, Beta adaptin 1, CLAPB2 |
| Calculated Molecular Weight | 919 aa, 101 kDa |
| Observed Molecular Weight | 101 kDa |
| GenBank Accession Number | BC046242 |
| Gene Symbol | AP1B1 |
| Gene ID (NCBI) | 162 |
| RRID | AB_2058204 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q10567 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
AP1B1 is a subunit of the adaptor complex AP-1 and is a member of the adaptin protein family. Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP-1 is found at the cytoplasmic face of coated vesicles located at the Golgi complex, where it mediates both the recruitment of clathrin to the membrane and the recognition of sorting signals within the cytosolic tails of transmembrane receptors.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for AP1B1 antibody 16932-1-AP | Download protocol |
| IF protocol for AP1B1 antibody 16932-1-AP | Download protocol |
| IP protocol for AP1B1 antibody 16932-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Genet Bidirectional genome-wide CRISPR screens reveal host factors regulating SARS-CoV-2, MERS-CoV and seasonal HCoVs | ||
J Cell Biol Cargo-selective SNX-BAR proteins mediate retromer trimer independent retrograde transport. | ||
Oncogene Regulated secretion of mutant p53 negatively affects T lymphocytes in the tumor microenvironment | ||
Sci Adv ER-export and ARFRP1/AP-1-dependent delivery of SARS-CoV-2 Envelope to lysosomes controls late stages of viral replication |
