验证数据展示
经过测试的应用
| Positive WB detected in | mouse colon tissue, Hacat cells |
| Positive IHC detected in | human pancreas cancer tissue, human kidney tissue, human bowen disease Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
10618-1-AP targets AP1M2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Immunogen | AP1M2 fusion protein Ag0961 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | adaptor-related protein complex 1, mu 2 subunit |
| Synonyms | AP1 mu2, AP-1 complex subunit mu-2, AP mu chain family member mu1B, AP 1 complex subunit mu 2, Adaptor-related protein complex 1 subunit mu-2 |
| Calculated Molecular Weight | 48 kDa |
| Observed Molecular Weight | 50-55 kDa |
| GenBank Accession Number | BC005021 |
| Gene Symbol | AP1M2 |
| Gene ID (NCBI) | 10053 |
| RRID | AB_2242895 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y6Q5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
AP1M2 is a subunit of the adaptor complex AP-1. It belongs to the adaptor complexes medium subunit family. Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP-1 is found at the cytoplasmic face of coated vesicles located at the Golgi complex, where it mediates both the recruitment of clathrin to the membrane and the recognition of sorting signals within the cytosolic tails of transmembrane receptors.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for AP1M2 antibody 10618-1-AP | Download protocol |
| IHC protocol for AP1M2 antibody 10618-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
