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ATP1A3 (middle) Polyclonal antibody

ATP1A3 (middle) Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 28030-1-AP

Print datasheet

Synonyms

ATP1A3, ATP1A3 (middle), DYT12, RDP, Sodium pump subunit alpha 3

验证数据展示

  • IF Staining of HeLa using 28030-1-AP

    IF Staining of HeLa using 28030-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 28030-1-AP (ATP1A3 (middle) antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • WB analysis of HeLa using 28030-1-AP

    WB analysis of HeLa using 28030-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 28030-1-AP (ATP1A3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 28030-1-AP

    WB analysis using 28030-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28030-1-AP (ATP1A3 (middle) antibody) at dilution of 1:14000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 28030-1-AP

    IHC staining of human prostate cancer using 28030-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28030-1-AP (ATP1A3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ATP1A3 antibody included

经过测试的应用

Positive WB detected inHeLa cells, SH-SY5Y cells, SKOV-3 cells, U-87 MG cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ATP1A3 Ready-To-Use IHC Kit. ATP1A3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28030-1-AP targets ATP1A3 (middle) in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity Human
Cited Reactivityhuman
Immunogen ATP1A3 (middle) fusion protein Ag27705 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ATPase, Na+/K+ transporting, alpha 3 polypeptide
Synonyms ATP1A3, ATP1A3 (middle), DYT12, RDP, Sodium pump subunit alpha 3
Calculated Molecular Weight 113 kDa
Observed Molecular Weight 113 kDa
GenBank Accession NumberBC015566
Gene Symbol ATP1A3
Gene ID (NCBI) 478
RRIDAB_2881041
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP13637
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATP1A3 participates in the catalyticing hydrolysis of ATP and the exchanging of sodium and potassium ions across plasma membrane. The catalyticing activity mode is ATP + H2O + Na+(In) + K+(Out) = ADP + phosphate + Na+(Out) + K+(In). It has been published that the neurologic disorders rapid-onset dystonia-parkionsonism (RDP), alternating hemiplegia of childhood (ACH) and CAPOS syndrome (cerebellar ataxia, areflexia, pes cavus, optic atrophy and sensorineural hearing loss) are all related with the mutation of ATP1A3. There are other reports suggest that early life epilepsy and episodic apnea revealing are potentially associated with the mutation of ATP1A3 as a result of impairment of Na+/K+ homeostasis. This antibody is generated agaist the middle region (378-580aa) of ATP1A3 and detects 113 kDa protein in SDS-PAGE.(PMID: 30097153, 20301294, 29922587)

实验方案

Product Specific Protocols
WB protocol for ATP1A3 (middle) antibody 28030-1-APDownload protocol
IHC protocol for ATP1A3 (middle) antibody 28030-1-APDownload protocol
IF protocol for ATP1A3 (middle) antibody 28030-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB, IF

Sci Adv

De novo ATP1A3 variants cause polymicrogyria.

Authors - Satoko Miyatake
View Article
humanWB

Nat Commun

Homophilic ATP1A1 binding induces activin A secretion to promote EMT of tumor cells and myofibroblast activation.

Authors - Yi-Ing Chen
View Article