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  • Featured Product
  • KD/KO Validated

ATPIF1 Polyclonal antibody

ATPIF1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 12067-1-AP

Print datasheet

Synonyms

IF(1), ATPIP, ATPI, ATPase inhibitory factor 1, ATP5IF1

验证数据展示

  • WB analysis of HeLa using 12067-1-AP

    WB analysis of HeLa using 12067-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12067-1-AP (ATPIF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 12067-1-AP

    IHC staining of human liver using 12067-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12067-1-AP (ATPIF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of HeLa using 12067-1-AP

    IP experiment of HeLa using 12067-1-AP

    IP result of anti-ATPIF1 (IP:12067-1-AP, 4ug; Detection:12067-1-AP 1:500) with HeLa cells lysate 3440ug.

  • IF Staining of HepG2 using 12067-1-AP

    IF Staining of HepG2 using 12067-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ATPIF1 antibody (12067-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12067-1-AP targets ATPIF1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human
Cited Reactivityhuman
Immunogen ATPIF1 fusion protein Ag2704 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ATPase inhibitory factor 1
Synonyms IF(1), ATPIP, ATPI, ATPase inhibitory factor 1, ATP5IF1
Calculated Molecular Weight 106 aa, 12 kDa
Observed Molecular Weight 12 kDa
GenBank Accession NumberBC009677
Gene Symbol ATPIF1
Gene ID (NCBI) 93974
RRIDAB_2063159
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UII2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The H(+)-ATP synthase is a reversible engine of mitochondria that synthesizes or hydrolyzes ATP upon changes in cell physiology. ATP synthase dysfunction is involved in the onset and progression of diverse human pathologies. ATPIF1 gene encodes mitochondrial ATPase Inhibitory Factor 1 (IF1), also named ATPI, ATPIP or IP. Endogenous IF1 limits ATP depletion when the mitochondrial membrane potential falls below a threshold and the ATP synthase starts hydrolyzing ATP to pump protons out of the mitochondrial matrix. Mitochondrial content of IF1 controls the activity of oxidative phosphorylation mediating the shift of cancer cells to an enhanced aerobic glycolysis, thus supporting an oncogenic role of IF1 in cancer.

实验方案

Product Specific Protocols
WB protocol for ATPIF1 antibody 12067-1-APDownload protocol
IHC protocol for ATPIF1 antibody 12067-1-APDownload protocol
IF protocol for ATPIF1 antibody 12067-1-APDownload protocol
IP protocol for ATPIF1 antibody 12067-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Nat Biotechnol

Magnify is a universal molecular anchoring strategy for expansion microscopy

Authors - Aleksandra Klimas
View Article
IF

Br J Pharmacol

The compound BTB06584 is an IF1 -dependent selective inhibitor of the mitochondrial F1 Fo-ATPase.

Authors - Fabrice Ivanes
View Article
humanIF,WB

Cell Biosci

ATPIF1 maintains normal mitochondrial structure which is impaired by CCM3 deficiency in endothelial cells.

Authors - Kang Wang
View Article
WB,IF

Lab Invest

ATP synthase inhibitory factor subunit 1 regulates islet β-cell function via repression of mitochondrial homeostasis

Authors - Kailiang Zhang
  • KD Validated
View Article
WB,IF

Lab Invest

ATP synthase inhibitory factor subunit 1 regulates islet β-cell function via repression of mitochondrial homeostasis.

Authors - Kailiang Zhang
  • KD Validated
View Article
IHC,IF

Front Physiol

F1F0-ATP Synthase Inhibitory Factor 1 in the Normal Pancreas and in Pancreatic Ductal Adenocarcinoma: Effects on Bioenergetics, Invasion and Proliferation.

Authors - Helen Tanton
View Article