验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy AURKA Ready-To-Use IHC Kit. AURKA primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66757-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-66757 targets AURKA in IF applications and shows reactivity with Human, Mouse, Rat samples.
| Tested Applications | IF Application Description |
| Tested Reactivity | Human, Mouse, Rat |
| Immunogen | AURKA fusion protein Ag20096 种属同源性预测 |
| Host / Isotype | Mouse / IgG3 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | aurora kinase A |
| Synonyms | AIK, ARK 1, ARK1, AURA, AURKA, aurora kinase A, Aurora related kinase 1, Aurora/IPL1 related kinase 1, AURORA2, Breast tumor amplified kinase, BTAK, hARK1, STK15, STK6, STK7 |
| Calculated Molecular Weight | 46 kDa |
| Observed Molecular Weight | 46-52 kDa |
| GenBank Accession Number | BC002499 |
| Gene Symbol | AURKA |
| Gene ID (NCBI) | 6790 |
| RRID | AB_2883624 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O14965 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
AURKA(Aurora kinase A), also named as STK6, STK15 or AIK, belongs to the Ser/Thr protein kinase family. This protein may play a role in cell cycle regulation during anaphase and/or telophase by being involved in microtubule formation and/or stabilization. Some recent evidence revealed that AURKA may also be implicated in tumor development and progression. AURKA is expressed highly in testis and various proliferating cells, migrating as a 46 kDa protein in SDS PAGE.Nuclear staining for AURKA is weak or nonexistent in normal tissue but strong in tumor tissue(PMID:19107951).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 AURKA antibody CL594-66757 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |