验证数据展示
经过测试的应用
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL594-66757 targets AURKA in IF applications and shows reactivity with Human, Mouse, Rat samples.
Tested Applications | IF Application Description |
Tested Reactivity | Human, Mouse, Rat |
Immunogen | AURKA fusion protein Ag20096 种属同源性预测 |
Host / Isotype | Mouse / IgG3 |
Class | Monoclonal |
Type | Antibody |
Full Name | aurora kinase A |
Synonyms | AIK, ARK 1, ARK1, AURA, AURKA, aurora kinase A, Aurora related kinase 1, Aurora/IPL1 related kinase 1, AURORA2, Breast tumor amplified kinase, BTAK, hARK1, STK15, STK6, STK7 |
Calculated Molecular Weight | 46 kDa |
Observed Molecular Weight | 46-52 kDa |
GenBank Accession Number | BC002499 |
Gene Symbol | AURKA |
Gene ID (NCBI) | 6790 |
RRID | AB_2883624 |
Conjugate | CoraLite®594 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | O14965 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
AURKA(Aurora kinase A), also named as STK6, STK15 or AIK, belongs to the Ser/Thr protein kinase family. This protein may play a role in cell cycle regulation during anaphase and/or telophase by being involved in microtubule formation and/or stabilization. Some recent evidence revealed that AURKA may also be implicated in tumor development and progression. AURKA is expressed highly in testis and various proliferating cells, migrating as a 46 kDa protein in SDS PAGE.Nuclear staining for AURKA is weak or nonexistent in normal tissue but strong in tumor tissue(PMID:19107951).
实验方案
Product Specific Protocols | |
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IF protocol for CL594 AURKA antibody CL594-66757 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |