AZI2/NAP1 Polyclonal antibody
AZI2/NAP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, IF/ICC, IP, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, HeLa cells |
Positive IP detected in | HEK-293 cells, HepG2 cells |
Positive IHC detected in | mouse testis tissue, human pancreas tissue, human stomach tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells, HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:10000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
15042-1-AP targets AZI2/NAP1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
Cited Applications | WB, IHC, IF |
Tested Reactivity | human |
Cited Reactivity | human |
Immunogen | AZI2/NAP1 fusion protein Ag6892 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | 5-azacytidine induced 2 |
Synonyms | AZI2, Nap1, Nak-associated protein 1, Nak associated protein 1, 5-azacytidine-induced protein 2 |
Calculated Molecular Weight | 45 kDa |
Observed Molecular Weight | 45 kDa |
GenBank Accession Number | BC001139 |
Gene Symbol | AZI2 |
Gene ID (NCBI) | 64343 |
RRID | AB_2878103 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9H6S1 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for AZI2/NAP1 antibody 15042-1-AP | Download protocol |
IHC protocol for AZI2/NAP1 antibody 15042-1-AP | Download protocol |
IF protocol for AZI2/NAP1 antibody 15042-1-AP | Download protocol |
IP protocol for AZI2/NAP1 antibody 15042-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cancer Res FIP200 suppresses immune checkpoint therapy responses in breast cancers by limiting AZI2/TBK1/IRF signaling independent of its canonical autophagy function. | ||
PLoS Pathog MAVS activates TBK1 and IKKε through TRAFs in NEMO dependent and independent manner. | ||
Front Immunol Increased IKKϵ protein stability ensures efficient type I interferon responses in conditions of TBK1 deficiency | ||
Autophagy AZI2 mediates TBK1 activation at unresolved selective autophagy cargo receptor complexes with implications for CD8 T-cell infiltration in breast cancer | ||
J Cell Biol NAK-associated protein 1/NAP1 activates TBK1 to ensure accurate mitosis and cytokinesis |