• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

BEND3 Polyclonal antibody

BEND3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 23101-1-AP

Print datasheet

Synonyms

BEN domain containing 3, BEND3, KIAA1553, RP11 59I9.2

验证数据展示

  • WB analysis using 23101-1-AP

    WB analysis using 23101-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 23101-1-AP (BEND3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 23101-1-AP

    IHC staining of human liver cancer using 23101-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 23101-1-AP (BEND3 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver cancer using 23101-1-AP

    IHC staining of human liver cancer using 23101-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 23101-1-AP (BEND3 Antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of HEK-293 using 23101-1-AP

    IF Staining of HEK-293 using 23101-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using BEND3 antibody (23101-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inHEK-293 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23101-1-AP targets BEND3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen BEND3 fusion protein Ag19137 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name BEN domain containing 3
Synonyms BEN domain containing 3, BEND3, KIAA1553, RP11 59I9.2
Calculated Molecular Weight 828 aa, 94 kDa
Observed Molecular Weight94 kDa
GenBank Accession NumberBC131628
Gene Symbol BEND3
Gene ID (NCBI) 57673
RRIDAB_2879210
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ5T5X7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BEND3 is a quadruple BEN domain-containing protein that associates with heterochromatin and functions as a transcriptional repressor. BEND3 is highly expressed in pluripotent cells, and the induction of differentiation results in the down-regulation of BEND3. (PMID: 35217604, PMID: 25600804)

实验方案

Product Specific Protocols
WB protocol for BEND3 antibody 23101-1-APDownload protocol
IHC protocol for BEND3 antibody 23101-1-APDownload protocol
IF protocol for BEND3 antibody 23101-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols