BHMT Polyclonal antibody

BHMT Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (3)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15965-1-AP

Print datasheet

Synonyms

BHMT



经过测试的应用

Positive WB detected inmouse kidney tissue, human kidney tissue, L02 cells
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman spleen tissue, human heart tissue, human lung tissue, human ovary tissue, human placenta tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy BHMT Ready-To-Use IHC Kit. BHMT primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15965-1-AP targets BHMT in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, pig, chicken
Immunogen BHMT fusion protein Ag8738 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name betaine-homocysteine methyltransferase
Synonyms BHMT
Calculated Molecular Weight 406 aa, 45 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC012616
Gene Symbol BHMT
Gene ID (NCBI) 635
RRIDAB_2290472
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ93088
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for BHMT antibody 15965-1-APDownload protocol
IHC protocol for BHMT antibody 15965-1-APDownload protocol
IP protocol for BHMT antibody 15965-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell

mTORC1 stimulates cell growth through SAM synthesis and m6A mRNA-dependent control of protein synthesis.

Authors - Elodie Villa
mouseWB

NPJ Sci Food

Ablation of the gut microbiota alleviates high-methionine diet-induced hyperhomocysteinemia and glucose intolerance in mice

Authors - Wenqiang Li
WB

J Anim Sci Biotechnol

Dietary betaine activates hepaticVTGIIexpression in laying hens associated with hypomethylation ofGRgene promoter and enhancedGRexpression.

Authors - Nagmeldin A Omer
ratWB

J Agric Food Chem

Mitotically stable modification of DNA methylation in IGF2/H19 imprinting control region is associated with activated hepatic IGF2 expression in offspring rats from betaine-supplemented dams.

Authors - Shu Yang
pigWB

J Agric Food Chem

Maternal Betaine Supplementation during Gestation Enhances Expression of mtDNA-Encoded Genes through D-Loop DNA Hypomethylation in the Skeletal Muscle of Newborn Piglets.

Authors - Yimin Jia
pigWB

Sci Rep

Abnormally activated one-carbon metabolic pathway is associated with mtDNA hypermethylation and mitochondrial malfunction in the oocytes of polycystic gilt ovaries.

Authors - Longfei Jia