验证数据展示
经过测试的应用
| Positive WB detected in | Daudi cells, HeLa cells, RAW 264.7 cells, U-937 cells, Raji cells |
| Positive IP detected in | Daudi cells |
| Positive IHC detected in | human tonsillitis tissue, human lung cancer tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 17 publications below |
| IHC | See 5 publications below |
产品信息
24304-1-AP targets cIAP2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | cIAP2 fusion protein Ag20143 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | baculoviral IAP repeat-containing 3 |
| 别名 | BIRC3, CIAP 2, Cellular inhibitor of apoptosis 2, C IAP2, BIRC 3 |
| 计算分子量 | 604 aa, 68 kDa |
| 观测分子量 | 68 kDa |
| GenBank蛋白编号 | BC027485 |
| 基因名称 | cIAP2 |
| Gene ID (NCBI) | 330 |
| RRID | AB_2879485 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q13489 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
cIAP2 also known as BIRC3, is a member of the Inhibitor of Apoptosis Protein (IAP) family. cIAP2 is induced by inflammatory stimuli and plays a critical role in regulating adaptive and innate immune responses, cell death, and the production of inflammatory mediators. Its dysregulation is associated with various diseases, including cancer and neuroinflammatory conditions. Understanding the mechanisms by which cIAP2 functions and interacts with other cellular components is essential for developing targeted therapies.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for cIAP2 antibody 24304-1-AP | Download protocol |
| IHC protocol for cIAP2 antibody 24304-1-AP | Download protocol |
| IP protocol for cIAP2 antibody 24304-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Dev Cell Selective Autophagy of Mitochondria on a Ubiquitin-Endoplasmic-Reticulum Platform. | ||
Oncotarget The putative tumor activator ARHGEF3 promotes nasopharyngeal carcinoma cell pathogenesis by inhibiting cellular apoptosis. | ||
Front Oncol Transcriptome-Based Network Analysis Unveils Eight Immune-Related Genes as Molecular Signatures in the Immunomodulatory Subtype of Triple-Negative Breast Cancer. | ||
Biomed Pharmacother Chlorogenic acid inhibits the proliferation of human lung cancer A549 cell lines by targeting annexin A2 in vitro and in vivo. | ||
Cancer Med Targeting inhibitors of apoptosis proteins suppresses medulloblastoma cell proliferation via G2/M phase arrest and attenuated neddylation of p21. | ||
Cancer Biol Ther Survivin knockdown combined with apoptin overexpression inhibits cell growth significantly. |
