验证数据展示
经过测试的应用
| Positive WB detected in | L02 cells, human blood, mouse liver tissue |
| Positive IP detected in | L02 cells |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:4000-1:40000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
22233-1-AP targets C4 Alpha Chain/C4b/C4d in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, CoIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | C4 Alpha Chain/C4b/C4d fusion protein Ag17583 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | complement component 4A (Rodgers blood group) |
| 别名 | Acidic complement C4, C4, C4A, C4A2, C4A3, C4A4, C4A6, C4S, CO4, Complement C4 A, CPAMD2, RG |
| 计算分子量 | 1744 aa, 193 kDa |
| 观测分子量 | 80-95 kDa, 200 kDa |
| GenBank蛋白编号 | BC063289 |
| 基因名称 | C4 Gamma Chain |
| Gene ID (NCBI) | 720 |
| RRID | AB_2879042 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P0C0L4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Complement C4, a component of the classical complement pathway, has an important role in innate immune function. C4 protein is expressed as a single chain precursor which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains (molecular wights 95, 78, and 31 kDa) prior to secretion. The alpha chain is cleaved to release C4 anaphylatoxin, an antimicrobial peptide and a mediator of local inflammation. This antibody raised against 1017-1336aa of human C4-A protein can recognize C4 precursor, C4 alpha chain, C4b, and C4d.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for C4 Alpha Chain/C4b/C4d antibody 22233-1-AP | Download protocol |
| IHC protocol for C4 Alpha Chain/C4b/C4d antibody 22233-1-AP | Download protocol |
| IP protocol for C4 Alpha Chain/C4b/C4d antibody 22233-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Free Radic Biol Med Complement components regulates ferroptosis in CVB3 viral myocarditis by interatction with TFRC
| ||
Cell Biochem Funct In silico analysis and verification of critical genes related to vascular calcification in multiple diseases | ||
Immunobiology Inefficient and abortive classical complement pathway activation by the calcium inositol hexakisphosphate component of the Echinococcus granulosus laminated layer. | ||
Transplant Direct Circulating Donor Heart Exosome Profiling Enables Noninvasive Detection of Antibody-mediated Rejection. | ||
