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验证数据展示

  • WB analysis of mouse brain using 11443-1-AP

    WB analysis of mouse brain using 11443-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 11443-1-AP (CA IX antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of A549 using 11443-1-AP

    WB analysis of A549 using 11443-1-AP

    WB result of CA9 antibody (11443-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CA9 transfected A549 cells.

  • IHC staining of human stomach cancer using 11443-1-AP

    IHC staining of human stomach cancer using 11443-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11443-1-AP (CA9 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CA9 antibody included
  • Featured Product
  • KD/KO Validated

CA9 Polyclonal antibody

CA9 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11443-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

CA IX, CA9, CAIX, Carbonate dehydratase IX, Carbonic anhydrase 9, carbonic anhydrase IX, G250, Membrane antigen MN, MN, P54/58N, pMW1, RCC associated antigen G250

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, A549 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CA9 Ready-To-Use IHC Kit. CA9 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11443-1-AP targets CA9 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen CA9 fusion protein Ag1963 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name carbonic anhydrase IX
Synonyms CA IX, CA9, CAIX, Carbonate dehydratase IX, Carbonic anhydrase 9, carbonic anhydrase IX, G250, Membrane antigen MN, MN, P54/58N, pMW1, RCC associated antigen G250
Calculated Molecular Weight 459 aa, 50 kDa
Observed Molecular Weight 50-60 kDa
GenBank Accession NumberBC014950
Gene Symbol CA9
Gene ID (NCBI) 768
RRIDAB_2243631
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16790
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CA9(Carbonic anhydrase 9) may be involved in the control of cell proliferation and transformation and appears to be a novel specific biomarker for a cervical neoplasia(PMID:18703501).It is a tumor-associated antigen that has been shown to have diagnostic utility in identifying cervical dysplasia and carcinoma.The protein is presentboth on the plasma membrane and in the nucleus of cells and has the molecular.

实验方案

Product Specific Protocols
WB protocol for CA9 antibody 11443-1-APDownload protocol
IHC protocol for CA9 antibody 11443-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols