验证数据展示
经过测试的应用
| Positive WB detected in | SMMC-7721 cells, human brain tissue |
| Positive IP detected in | mouse heart tissue |
| Positive IHC detected in | mouse heart tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12666-2-AP targets CaMKII Gamma in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | CaMKII Gamma fusion protein Ag3348 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | calcium/calmodulin-dependent protein kinase II gamma |
| 别名 | CAMK2G, CaMK-II subunit gamma, CAMK-II, CAMK, CaM kinase II subunit gamma |
| 计算分子量 | 527 aa, 59 kDa |
| 观测分子量 | 59 kDa |
| GenBank蛋白编号 | BC034044 |
| 基因名称 | CAMK2G |
| Gene ID (NCBI) | 818 |
| RRID | AB_2068213 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q13555 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CaMKII gamma, also named CAMKG or CAMK2G, belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family, and CaMK subfamily. CAMK2 is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. CAMK2G is expressed in human beta cells as a candidate gene for Type II (non-insulin-dependent) diabetes mellitus. Some studies can be used to assess the role of CAMK2G in the susceptibility to Type II diabetes(PMID: 12032636). CAMK2G has some isoforms with the MW of 55-66 kDa and this antibody can recognize all the isoforms of CAMK2G. This antibody may have cross-reaction with CAMK2A/B/D due to the high homology.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CaMKII Gamma antibody 12666-2-AP | Download protocol |
| IHC protocol for CaMKII Gamma antibody 12666-2-AP | Download protocol |
| IF protocol for CaMKII Gamma antibody 12666-2-AP | Download protocol |
| IP protocol for CaMKII Gamma antibody 12666-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Neuron JAKMIP1, a Novel Regulator of Neuronal Translation, Modulates Synaptic Function and Autistic-like Behaviors in Mouse. | ||
Transl Res Cardiac-Specific Overexpression of CREM-IbΔC-X via CRISPR/Cas9 in Mice Presents a New Model of Atrial Cardiomyopathy with Spontaneous Atrial Fibrillation | ||
Oncotarget PIWIL1 destabilizes microtubule by suppressing phosphorylation at Ser16 and RLIM-mediated degradation of Stathmin1. | ||
Development Calmodulin-dependent protein kinase gamma 3 (CamKIIgamma3) mediates the cell cycle resumption of metaphase II eggs in mouse. | ||
Aging (Albany NY) CAMKIIγ is a targetable driver of multiple myeloma through CaMKIIγ/ Stat3 axis.
| ||
Cell Commun Signal Wnt5a-induced M2 polarization of tumor-associated macrophages via IL-10 promotes colorectal cancer progression. |
