验证数据展示
经过测试的应用
| Positive WB detected in | human cerebellum tissue, mouse thymus tissue |
| Positive IHC detected in | human ovary cancer tissue, human brain tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2400 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 5 publications below |
| IF | See 2 publications below |
产品信息
13263-1-AP targets CaMKIV in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | CaMKIV fusion protein Ag3922 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | calcium/calmodulin-dependent protein kinase IV |
| 别名 | CAMK4, CaMK IV, CaMK GR, CAMK, CaM kinase-GR |
| 计算分子量 | 473 aa, 52 kDa |
| 观测分子量 | 56-65 kDa |
| GenBank蛋白编号 | BC025687 |
| 基因名称 | CAMK4 |
| Gene ID (NCBI) | 814 |
| RRID | AB_2068381 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q16566 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CaMK4 (Calcium/calmodulin-dependent protein kinase IV) is a multifunctional serine/threonine kinase that regulates gene expression by activating transcription factors in a wide range of immune cells including T cells and antigen-presenting cells. The function of CaMK4 is suggested to be abnormal mainly in systemic lupus erythematosus (SLE), which is characterized by autoantibody production, immune complex formation, and immune dysregulation (PMID: 29252071). CaMKIV can translocate between the nucleus and cytoplasm (PMID: 21301225)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CaMKIV antibody 13263-1-AP | Download protocol |
| IHC protocol for CaMKIV antibody 13263-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Oncotarget PIWIL1 destabilizes microtubule by suppressing phosphorylation at Ser16 and RLIM-mediated degradation of Stathmin1. | ||
J Invest Dermatol The Long Noncoding RNA UCA1 Negatively Regulates Melanogenesis in Melanocytes. | ||
Toxicol Res (Camb) Neurotoxicity, behavioral changes and gene-expression profile of mice exposed to SnS2 nanoflowers. | ||
Cell Death Discov Congenital hypothyroidism impairs spine growth of dentate granule cells by downregulation of CaMKIV. | ||
Front Mol Biosci Novel cuproptosis metabolism-related molecular clusters and diagnostic signature for Alzheimer's disease | ||
Fitoterapia Investigation of the underlying mechanism of Buyang Huanwu decoction in ischemic stroke by integrating systems pharmacology-proteomics and in vivo experiments |
