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验证数据展示

  • WB analysis of mouse brain using 27106-1-AP

    WB analysis of mouse brain using 27106-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 27106-1-AP (CCDC124 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 27106-1-AP

    IF Staining of HeLa using 27106-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 27106-1-AP (CCDC124 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

CCDC124 Polyclonal antibody

CCDC124 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 27106-1-AP

Print datasheet

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA and More (1)

Synonyms

CCDC124

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, human placenta tissue, mouse liver tissue
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27106-1-AP targets CCDC124 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen CCDC124 fusion protein Ag25930 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name coiled-coil domain containing 124
Synonyms CCDC124
Observed Molecular Weight 30-32 kDa
GenBank Accession NumberBC013949
Gene Symbol CCDC124
Gene ID (NCBI) 115098
RRIDAB_2880758
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96CT7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CCDC124 is a novel centrosomal and midbody protein that is highly conserved among species. It is ubiquitously expressed with high expression in brain, placenta, liver, spleen, and prostate. At telophase, CCDC124 dissociates from centrosomes and relocalizes to the midzone, subsequently accumulating at the midbody at cytokinesis. It interacts with the Ras-guanine nucleotide exchange factor 1B (RasGEF1B) and is involved in cytokinesis.

实验方案

Product Specific Protocols
WB protocol for CCDC124 antibody 27106-1-APDownload protocol
IF protocol for CCDC124 antibody 27106-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Protein J

Live Cell Protein Imaging of Tandem Complemented-GFP11-Tagged Coiled-Coil Domain-Containing Protein-124 Identifies this Factor in G3BP1-Induced Stress-Granules

Authors - Kübra Hacibeyoğlu
View Article