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验证数据展示

  • IHC staining of human testis using 22301-1-AP

    IHC staining of human testis using 22301-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 22301-1-AP (CCL27 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human testis using 22301-1-AP

    IHC staining of human testis using 22301-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 22301-1-AP (CCL27 Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of A431 using 22301-1-AP

    IF Staining of A431 using 22301-1-AP

    Immunofluorescent analysis of A431 cells using 22301-1-AP (CCL27 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

CCL27 Polyclonal antibody

CCL27 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 22301-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Synonyms

ALP, C C motif chemokine 27, CC chemokine ILC, CCL27, CTACK, CTAK, ESKINE, IL 11 R alpha locus chemokine, ILC, PESKY, SCYA27, Skinkine, Small inducible cytokine A27

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive IHC detected inhuman testis tissue, human liver tissue, human liver cancer tissue, human skin tissue, human skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22301-1-AP targets CCL27 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chemokine (C-C motif) ligand 27
Synonyms ALP, C C motif chemokine 27, CC chemokine ILC, CCL27, CTACK, CTAK, ESKINE, IL 11 R alpha locus chemokine, ILC, PESKY, SCYA27, Skinkine, Small inducible cytokine A27
Calculated Molecular Weight 13 kDa
GenBank Accession NumberNM_006664
Gene Symbol CCL27
Gene ID (NCBI) 10850
RRIDAB_2879065
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y4X3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CCL27, also known as CTACK, is a member of the CC chemokine subfamily. CCL27 is predominantly expressed in skin and selectively attracts cutaneous lymphocyte antigen-positive T cells and Langerhans cells. The CCL27 secreted from keratinocytes was reportedly involved in inflammatory skin diseases such as atopic dermatitis, contact dermatitis, and psoriasis. CCL27 is a ligand to CCR10. The binding of CCL27 to CCR10 on T cells promotes their migration to the skin, where they can then participate in local immune responses.

实验方案

Product Specific Protocols
IHC protocol for CCL27 antibody 22301-1-APDownload protocol
IF protocol for CCL27 antibody 22301-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols