验证数据展示
经过测试的应用
Positive IF-P detected in | human tonsillitis tissue, human placenta tissue |
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
产品信息
CL594-66065 targets CD31 in WB, IF-P applications and shows reactivity with human samples.
Tested Applications | IF-P Application Description |
Cited Applications | WB |
Tested Reactivity | human |
Cited Reactivity | human |
Immunogen | CD31 fusion protein Ag1787 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | platelet/endothelial cell adhesion molecule |
Synonyms | PECAM 1, PECA1, GPIIA', GPIIA, EndoCAM |
Calculated Molecular Weight | 83 kDa |
GenBank Accession Number | BC022512 |
Gene Symbol | CD31 |
Gene ID (NCBI) | 5175 |
ENSEMBL Gene ID | ENSG00000261371 |
RRID | AB_2883500 |
Conjugate | CoraLite®594 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P16284 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).This antibody is conjugated with CL594, Ex/Em 593 nm/614 nm.
实验方案
Product Specific Protocols | |
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IF protocol for CL594 CD31 antibody CL594-66065 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |