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验证数据展示

  • WB analysis using 55161-1-AP

    WB analysis using 55161-1-AP

    Human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 55161-1-AP (CD42c antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

CD42c Polyclonal antibody

CD42c Polyclonal Antibody for WB, ELISA
Cat No. 55161-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

GP1BB, GPIbB, GPIb beta, GP Ib beta, Antigen CD42b beta

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHuman peripheral blood platelets

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55161-1-AP targets CD42c in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name glycoprotein Ib (platelet), beta polypeptide
Synonyms GP1BB, GPIbB, GPIb beta, GP Ib beta, Antigen CD42b beta
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 26 kDa
GenBank Accession NumberNM_000407
Gene Symbol CD42c
Gene ID (NCBI) 2812
RRIDAB_2881279
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP13224
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD42c, also known as platelet glycoprotein Ib beta chain (GP1BB), is a transmembrane protein containing a leucine-rich amino acid sequence (PMID: 3353370). CD42c is covalently linked to platelet glycoprotein Ib alpha chain (CD42b/GP1BA) to form GPIb, which is part of the Glycoprotein Ib-V-IX receptor (GPIb-V-IX) complex that constitutes the receptor for von Willebrand factor (VWF), and mediates platelet adhesion (PMID: 7660135). Mutations in the GP1BB gene have been associated with Bernard-Soulier syndrome, velocardiofacial syndrome, and giant platelet disorder.

实验方案

Product Specific Protocols
WB protocol for CD42c antibody 55161-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols